Coupled high-throughput functional screening and next generation sequencing for identification of plant polymer decomposing enzymes in metagenomic libraries

Autor: Mari eNyyssönen, Huu M. Tran, Ulas eKaraoz, Claudia eWeihe, Masood Z. Hadi, Jennifer B. H. Martiny, Adam C. Martiny, Eoin L. Brodie

Publikováno v: Frontiers in Microbiology, Vol 4 (2013)

Recent advances in sequencing technologies generate new predictions and hypotheses about the functional roles of environmental microorganisms. Yet, until we can test these predictions at a scale that matches our ability to generate them, most of them

Externí odkaz: https://doaj.org/article/0613d62ed5964bcc9852286cb0e63a1f

Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes.

Autor: Ditte Hededam Welner, David Shin, Giovani P Tomaleri, Andy M DeGiovanni, Alex Yi-Lin Tsai, Huu M Tran, Sara Fasmer Hansen, Derek T Green, Henrik V Scheller, Paul D Adams

Publikováno v: PLoS ONE, Vol 12, Iss 6, p e0177591 (2017)

Molecular characterization of plant cell wall glycosyltransferases is a critical step towards understanding the biosynthesis of the complex plant cell wall, and ultimately for efficient engineering of biofuel and agricultural crops. The majority of t

Externí odkaz: https://doaj.org/article/9f8f4e863c084b8b9a054606ff1b6216

Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass.

Autor: Zhiwei Chen, Jose H Pereira, Hanbin Liu, Huu M Tran, Nathan S Y Hsu, Dean Dibble, Seema Singh, Paul D Adams, Rajat Sapra, Masood Z Hadi, Blake A Simmons, Kenneth L Sale

Publikováno v: PLoS ONE, Vol 8, Iss 11, p e79725 (2013)

Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification. However, even under these improved saccharification conditions th

Externí odkaz: https://doaj.org/article/5cb8e4835bd74f66bd11a779f46be48a

Automated 'Cells-To-Peptides' Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes

Autor: Joel M. Guenther, Nathan J. Hillson, Zak Costello, Christopher J. Petzold, Huu M. Tran, Leanne Jade G. Chan, Jacquelyn M. Blake-Hedges, Tadeusz L. Ogorzalek, Paul D. Adams, Jennifer W. Gin, Yan Chen, Jay D. Keasling, Hector Garcia Martin

Publikováno v: Journal of proteome research, vol 18, iss 10
Chen, Y, Guenther, J M, Gin, J W, Chan, L J G, Costello, Z, Ogorzalek, T L, Tran, H M, Blake-Hedges, J M, Keasling, J D, Adams, P D, García Martín, H, Hillson, N J & Petzold, C J 2019, ' Automated "cells-To-Peptides" Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes ', Journal of Proteome Research, vol. 18, no. 10, pp. 3752-3761 . https://doi.org/10.1021/acs.jproteome.9b00455

Mass spectrometry-based quantitative proteomic analysis has proven valuable for clinical and biotechnology-related research and development. Improvements in sensitivity, resolution, and robustness of mass analyzers have also added value. However, man

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::246503ddb83be4fd61c2365e38ce44d7
https://escholarship.org/uc/item/69q756zj

Plant cell wall glycosyltransferases: Highthroughput recombinant expression screening and general requirements for these challenging enzymes

Autor: Giovani P. Tomaleri, Paul D. Adams, Huu M. Tran, Andy DeGiovanni, Sara Fasmer Hansen, Derek Green, Ditte Hededam Welner, Henrik Vibe Scheller, Alex Yi-Lin Tsai, David S. Shin

Publikováno v: Welner, DH; Shin, D; Tomaleri, GP; DeGiovanni, AM; Yi-Lin Tsai, A; Tran, HM; et al.(2017). Plant cell wall glycosyltransferases: Highthroughput recombinant expression screening and general requirements for these challenging enzymes. PLoS ONE, 12(6). doi: 10.1371/journal.pone.0177591. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2b53r12k
PloS one, vol 12, iss 6
Welner, D H, Shin, D, Tomaleri, G P, DeGiovanni, A M, Tsai, A Y-L, Tran, H M, Hansen, S F, Green, D T, Scheller, H V, Adams, P D & Hofmann, A (ed.) 2017, ' Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes ', P L o S One, vol. 12, no. 6, 0177591 . https://doi.org/10.1371/journal.pone.0177591
PLoS ONE
PLoS ONE, Vol 12, Iss 6, p e0177591 (2017)

Molecular characterization of plant cell wall glycosyltransferases is a critical step towards understanding the biosynthesis of the complex plant cell wall, and ultimately for efficient engineering of biofuel and agricultural crops. The majority of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1094264b00cccf1d1cff9212153609a
http://www.escholarship.org/uc/item/2b53r12k