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Akademický článek

Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes.

Autor: Ditte Hededam Welner, David Shin, Giovani P Tomaleri, Andy M DeGiovanni, Alex Yi-Lin Tsai, Huu M Tran, Sara Fasmer Hansen, Derek T Green, Henrik V Scheller, Paul D Adams

Publikováno v: PLoS ONE, Vol 12, Iss 6, p e0177591 (2017)

Molecular characterization of plant cell wall glycosyltransferases is a critical step towards understanding the biosynthesis of the complex plant cell wall, and ultimately for efficient engineering of biofuel and agricultural crops. The majority of t

Externí odkaz: https://doaj.org/article/9f8f4e863c084b8b9a054606ff1b6216

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Akademický článek

Cellulosic biomass pretreatment and sugar yields as a function of biomass particle size.

Autor: Michael J Dougherty, Huu M Tran, Vitalie Stavila, Bernhard Knierim, Anthe George, Manfred Auer, Paul D Adams, Masood Z Hadi

Publikováno v: PLoS ONE, Vol 9, Iss 6, p e100836 (2014)

Three lignocellulosic pretreatment techniques (ammonia fiber expansion, dilute acid and ionic liquid) are compared with respect to saccharification efficiency, particle size and biomass composition. In particular, the effects of switchgrass particle

Externí odkaz: https://doaj.org/article/23e096223a994d1d9eda6c818c6e8ed3

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Akademický článek

Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass.

Autor: Zhiwei Chen, Jose H Pereira, Hanbin Liu, Huu M Tran, Nathan S Y Hsu, Dean Dibble, Seema Singh, Paul D Adams, Rajat Sapra, Masood Z Hadi, Blake A Simmons, Kenneth L Sale

Publikováno v: PLoS ONE, Vol 8, Iss 11, p e79725 (2013)

Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification. However, even under these improved saccharification conditions th

Externí odkaz: https://doaj.org/article/5cb8e4835bd74f66bd11a779f46be48a

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Efficient Prompt Scintillation and Fast Neutron-Gamma Ray Discrimination Using Amorphous Blends of Difluorenylsilane Organic Glass and In Situ Polymerized Vinyltoluene

Autor: Patrick L. Feng, Joseph S. Carlson, Huu M. Tran, Nicholas Myllenbeck, McKenzie A. Hunter, Annabelle I. Benin

Publikováno v: IEEE Transactions on Nuclear Science. 68:2400-2406

High-performance radiation detection materials are an integral part of national security, medical imaging, and nuclear physics applications. Those that offer compositional and manufacturing versatility are of particular interest. Here, we report a ne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3380396165e01754a81fbaac2f8ecb34
https://doi.org/10.1109/tns.2021.3096926

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Akademický článek

Coupled high-throughput functional screening and next generation sequencing for identification of plant polymer decomposing enzymes in metagenomic libraries

Autor: Mari eNyyssönen, Huu M. Tran, Ulas eKaraoz, Claudia eWeihe, Masood Z. Hadi, Jennifer B. H. Martiny, Adam C. Martiny, Eoin L. Brodie

Publikováno v: Frontiers in Microbiology, Vol 4 (2013)

Recent advances in sequencing technologies generate new predictions and hypotheses about the functional roles of environmental microorganisms. Yet, until we can test these predictions at a scale that matches our ability to generate them, most of them

Externí odkaz: https://doaj.org/article/0613d62ed5964bcc9852286cb0e63a1f

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Engineering glycoside hydrolase stability by the introduction of zinc binding

Autor: Joel M. Guenther, Andy DeGiovanni, Ryan P. McAndrew, Ditte Hededam Welner, Jose Henrique Pereira, Thomas L. Ellinghaus, Blake A. Simmons, Taya Feldman, Paul D. Adams, Kenneth L. Sale, Huu M. Tran

Publikováno v: Acta Crystallographica. Section D, Structural Biology

The engineering of metal binding into a cellulase increases its temperature stability while maintaining its other catalytic properties.
The development of robust enzymes, in particular cellulases, is a key step in the success of biological route

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23edb827995dc9529642e11a464f0691
https://doi.org/10.1107/s2059798318006678

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Plný text ve formátu HTML

Automated 'Cells-To-Peptides' Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes

Autor: Joel M. Guenther, Nathan J. Hillson, Zak Costello, Christopher J. Petzold, Huu M. Tran, Leanne Jade G. Chan, Jacquelyn M. Blake-Hedges, Tadeusz L. Ogorzalek, Paul D. Adams, Jennifer W. Gin, Yan Chen, Jay D. Keasling, Hector Garcia Martin

Publikováno v: Journal of proteome research, vol 18, iss 10
Chen, Y, Guenther, J M, Gin, J W, Chan, L J G, Costello, Z, Ogorzalek, T L, Tran, H M, Blake-Hedges, J M, Keasling, J D, Adams, P D, García Martín, H, Hillson, N J & Petzold, C J 2019, ' Automated "cells-To-Peptides" Sample Preparation Workflow for High-Throughput, Quantitative Proteomic Assays of Microbes ', Journal of Proteome Research, vol. 18, no. 10, pp. 3752-3761 . https://doi.org/10.1021/acs.jproteome.9b00455

Mass spectrometry-based quantitative proteomic analysis has proven valuable for clinical and biotechnology-related research and development. Improvements in sensitivity, resolution, and robustness of mass analyzers have also added value. However, man

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::246503ddb83be4fd61c2365e38ce44d7
https://escholarship.org/uc/item/69q756zj

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Plant cell wall glycosyltransferases: Highthroughput recombinant expression screening and general requirements for these challenging enzymes

Autor: Giovani P. Tomaleri, Paul D. Adams, Huu M. Tran, Andy DeGiovanni, Sara Fasmer Hansen, Derek Green, Ditte Hededam Welner, Henrik Vibe Scheller, Alex Yi-Lin Tsai, David S. Shin

Publikováno v: Welner, DH; Shin, D; Tomaleri, GP; DeGiovanni, AM; Yi-Lin Tsai, A; Tran, HM; et al.(2017). Plant cell wall glycosyltransferases: Highthroughput recombinant expression screening and general requirements for these challenging enzymes. PLoS ONE, 12(6). doi: 10.1371/journal.pone.0177591. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2b53r12k
PloS one, vol 12, iss 6
Welner, D H, Shin, D, Tomaleri, G P, DeGiovanni, A M, Tsai, A Y-L, Tran, H M, Hansen, S F, Green, D T, Scheller, H V, Adams, P D & Hofmann, A (ed.) 2017, ' Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes ', P L o S One, vol. 12, no. 6, 0177591 . https://doi.org/10.1371/journal.pone.0177591
PLoS ONE
PLoS ONE, Vol 12, Iss 6, p e0177591 (2017)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1094264b00cccf1d1cff9212153609a
http://www.escholarship.org/uc/item/2b53r12k

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