Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin

Autor: Weiyi Wang, Soraya Cantos-Fernandes, Yuncong Lv, Hureshitanmu Kuerban, Shoeb Ahmad, Chunguang Wang, Benoît Gigant

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)

Kinesin-13s are microtubule depolymerizing enzymes. Here the authors present the crystal structure of a DARPin fused construct comprising the short neck region and motor domain of kinesin-13 in complex with an αβ-tubulin heterodimer, which shows th

Externí odkaz: https://doaj.org/article/d77b0f1dd0244051951a29439456c549

New Insights into the Coupling between Microtubule Depolymerization and ATP Hydrolysis by Kinesin-13 Protein Kif2C

Autor: Benoît Gigant, Fuming Zhang, Marcel Knossow, Ting Shen, Yuncong Lv, Chunguang Wang, Hureshitanmu Kuerban, Raphael Guerois, Weiyi Wang

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2015, 290 (30), pp.18721-31. 〈10.1074/jbc.M115.646919〉
Journal of Biological Chemistry, 2015, 290 (30), pp.18721-31. ⟨10.1074/jbc.M115.646919⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2015, 290 (30), pp.18721-31. ⟨10.1074/jbc.M115.646919⟩

International audience; Kinesin-13 proteins depolymerize microtubules in an ATP hydrolysis-dependent manner. The coupling between these two activities remains unclear. Here, we first studied the role of the kinesin-13 subfamily-specific loop 2 and of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c07e7ba92e661f5fc78fbd5828b2ed69
https://hal.archives-ouvertes.fr/hal-01446760