GMOs: building the future on the basis of past experience

Autor: Luiz F.L. Reis, Marie-Anne Van Sluys, Richard C. Garratt, Humberto M. Pereira, Mauro M. Teixeira

Publikováno v: Anais da Academia Brasileira de Ciências, Vol 78, Iss 4, Pp 667-686 (2006)

Biosafety of genetically modified organisms (GMOs) and their derivatives is still a major topic in the agenda of government and societies worldwide. The aim of this review is to bring into light that data that supported the decision taken back in 199

Externí odkaz: https://doaj.org/article/5805d262324947d7bc0f3801bbcdfaec

Production in Pichia pastoris, antifungal activity and crystal structure of a class I chitinase from cowpea (Vigna unguiculata): Insights into sugar binding mode and hydrolytic action

Autor: Bruno Lopes de Sousa, Ana Cristina de Oliveira Monteiro-Moreira, Fredy D.A. Silva, Thalles B. Grangeiro, Humberto M. Pereira, Helen Paula Silva da Costa, José Brandão-Neto, Marina Duarte Pinto Lobo, Suelen Carneiro de Medeiros, Tuana Oliveira Correia, Jose T.A. Oliveira, Frederico Bruno Mendes Batista Moreno, Patrícia G. C. Landim, Cristina Paiva da Silveira Carvalho, Ito L. Barroso-Neto, Denise R. Nepomuceno, Valder N. Freire, Ilka M. Vasconcelos

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

A cowpea class I chitinase (VuChiI) was expressed in the methylotrophic yeast P. pastoris. The recombinant protein was secreted into the culture medium and purified by affinity chromatography on a chitin matrix. The purified chitinase migrated on SDS

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a34eb1c7a1ea460f898c64448f69c053
https://doi.org/10.1016/j.biochi.2017.01.014

Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases

Autor: José Brandão-Neto, Victoria Guixé, Felipe Gonzalez-Ordenes, Felipe Merino, Ricardo A. Zamora, Felipe Padilla-Salinas, Victor Castro-Fernandez, Humberto M. Pereira, Alejandra Herrera-Morande, Richard Charles Garratt

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ADP-dependent kinases of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4db92f4241622a50e70a7a9b04ec54db
https://europepmc.org/articles/PMC5743094/

Enzyme kinetics, structural analysis and molecular modeling studies on a series of Schistosoma mansoni PNP inhibitors

Autor: Renata Krogh, Marcela F. Terni, Humberto M. Pereira, Adriano D. Andricopulo, Matheus P. Postigo, Marcelo Santos Castilho, Glaucius Oliva

Publikováno v: Journal of the Brazilian Chemical Society v.22 n.3 2011
Journal of the Brazilian Chemical Society
Sociedade Brasileira de Química (SBQ)
instacron:SBQ
Journal of the Brazilian Chemical Society, Volume: 22, Issue: 3, Pages: 583-591, Published: MAR 2011
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

The enzyme purine nucleoside phosphorylase from Schistosoma mansoni (SmPNP) is an attractive molecular target for the development of novel drugs against schistosomiasis, a neglected tropical disease that affects about 200 million people worldwide. In

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::765a764389f1c3606750fc86fd0c76ea
https://doi.org/10.1590/s0103-50532011000300024

Crystal structure of Schistosoma purine nucleoside phosphorylase complexed with a novel monocyclic inhibitor

Autor: Anne Cleasby, Richard Charles Garratt, Valerio Berdini, Humberto M. Pereira, Mariana R. Ferri

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

A novel inhibitor of Schistosoma PNP was identified using an "in silico" approach allied to enzyme inhibition assays. The compound has a monocyclic structure which has not been previously described for PNP inhibitors. The crystallographic structure o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b525a8ec38ae7bd999ff8dac208326ec
https://doi.org/10.1016/j.actatropica.2010.01.010