Výsledky vyhledávání - "Huijbregts, Lukas"

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Additional file 10: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

Comparison of RIP and non-RIP retrovector expression in β and non-β cells. Non-β 293 T-TVA and MIN6-TVA insulinoma cells were transduced with the indicated retrovectors, in each case a pair of corresponding α- (RCANBP(A)) or SIN γ − (pSERS) re

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Additional file 8: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

Transgene expression level compared to that of two endogenous mRNA. Transgene expression level in populations of EndoC-βΗ2-TVA cells transduced with either RCANBP(A) RIP 3HAH2BYFP or pSERS RIP GFP-IRES-ZeoR (the same three populations as in Fig. 7)

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Additional file 7: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

Non-SIN α-retrovectors are not totally defective in mammalian cells. 293 T-TVA cells were transduced with the non-SIN α-retrovector RCAN CMV GFP-IRES-ZeoR and cultured 12 days in presence of zeocin. Then (that is 14 days and three passages after tr

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Additional file 4: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

BXV1 efficiently mobilizes non-SIN, but not SIN, γ-retrovector in doubly transduced EndoC-βΗ2 cells. A. EndoC-βΗ2 cells transduced with the non-SIN γ-retrovector pPRIHy-TVA (EndoC-βΗ2-TVA) were further transduced with a SIN γ-retrovector (pS

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Additional file 2: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

Stable EndoC-βΗ2 populations transduced with either pPRIZ or pSERS RIP γ-retrovector. The two stable populations described in Fig. 1 (EndoC-βΗ2 transduced with either pPRIZ GFP or pSERS RIP GFP) were continuously cultured in presence of zeocin f

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Additional file 9: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

Co-culture experiments to transduce TVA-expressing mammalian β cells with RCANBPB(A) RIP retrovector. DF1 chicken fibroblasts infected with RCAN RIP GFP-IRES-ZeoR were co-cultured with either EndoC-β Η2-TVA or MIN6-TVA cells (day 0). These two der

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Additional file 6: of New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines

Autor: Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël

RCANBP(A) RIP GFP-IRES-ZeoR transduced 293 T cells are resistant to zeocin thanks to non-RIP driven transcripts. A 293 T-TVA cells were transduced with the indicated α-retrovectors and cultured in presence of zeocin. The three retrovectors harbor Ze

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