Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Hui Theng Gan"'
Autor:
Muly Tham, Srinivas Ramasamy, Hui Theng Gan, Ashray Ramachandran, Anuradha Poonepalli, Yuan Hong Yu, Sohail Ahmed
Publikováno v:
PLoS ONE, Vol 5, Iss 12, p e15341 (2010)
Understanding how autocrine/paracrine factors regulate neural stem cell (NSC) survival and growth is fundamental to the utilization of these cells for therapeutic applications and as cellular models for the brain. In vitro, NSCs can be propagated alo
Externí odkaz:
https://doaj.org/article/63622e6287754d7e95ad8bd784a62b03
Autor:
Daniela Conti, Zachary Smiley, Jeff Carter, Melissa Stetson, Naresh Budhavaram, Jag Patel, Zaher Shabani, Janmeet Anant, Monica Cardona, Hui Theng Gan, James Hathcock, Jennifer Alejandre, Cecilia Turoff, Anderson Wong, Ken Wong, Isabelle Lequeux
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::54d8ca7e863f6b8c777e81e6a8796d92
https://doi.org/10.46220/2022reg003
https://doi.org/10.46220/2022reg003
Autor:
Rui Nian, Jeremy Lee, Hui Theng Gan, Pete Gagnon, Wei Zhang, Xeuzhi Bi, Lihan Tan, Yuansheng Yang
Publikováno v:
Journal of Chromatography A. 1431:1-7
Practical effects of advance chromatin removal on performance of protein A affinity chromatography were evaluated using a caprylic acid-allantoin-based extraction method. Lacking this treatment, the practice of increasing loading residence time to in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c16eee7602a37ed5c4cc7c0cf273c01
Autor:
Cindy Chuah, Pete Gagnon, Chiew Ling Lim, Rui Nian, Wei Zhang, Yuansheng Yang, Jeremy Lee, Hui Theng Gan, Xuezhi Bi, Lihan Tan, Sarah Maria Abdul Latiff
Publikováno v:
Journal of Chromatography A. 1340:68-78
Chromatin released from dead host cells during in vitro production of IgG monoclonal antibodies exists mostly in complex hetero-aggregates consisting of nucleosomal arrays (DNA+histone proteins), non-histone proteins, and aberrant forms of IgG. They
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36b04aff232a40538617a50891f96659
https://doi.org/10.1016/j.chroma.2014.03.010
https://doi.org/10.1016/j.chroma.2014.03.010
Autor:
Sarah Maria Abdul Latiff, Jeremy Lee, Hui Theng Gan, Phyllicia Toh, Cindy Chuah, Pete Gagnon, Wan Yee Lee
Publikováno v:
Journal of Chromatography A. 1291:33-40
We observed that IgM monoclonal antibodies and aggregates in mammalian cell culture supernatants were associated nonspecifically with nucleosomes, DNA, and histone proteins derived from nuclei of host cells that died during antibody production. A ser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c095dc721858f6016906687afa069548
https://doi.org/10.1016/j.chroma.2013.03.028
https://doi.org/10.1016/j.chroma.2013.03.028
Publikováno v:
Journal of Neurochemistry. 117:565-578
Neural stem cells (NSCs) are self-renewing multipotent cells that undergo symmetric and asymmetric cell division during development of the nervous system. The behavior of NSCs is tightly regulated by intrinsic processes such as transcriptional and po
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c48c1c933ab8610c9ea34b651f55ecda
https://doi.org/10.1111/j.1471-4159.2011.07227.x
https://doi.org/10.1111/j.1471-4159.2011.07227.x
Publikováno v:
Journal of chromatography. A. 1374
Chromatin heteroaggregates comprising nucleosomal arrays, DNA, histone and non-histone host cell proteins contributed 28% of the host contaminant mass in an IgG cell culture harvest. A subset comprising soluble particles of 50-400nm was retained thro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::928f320970bd25b066e26a91e2a14809
https://pubmed.ncbi.nlm.nih.gov/25481347
https://pubmed.ncbi.nlm.nih.gov/25481347
Autor:
Jeremy Lee, Hui Theng Gan, Yuansheng Yang, Wan Yee Lee, Cindy Chuah, Pete Gagnon, Rui Nian, Vincent Vagenende, Sarah Maria Abdul Latiff
Publikováno v:
Journal of chromatography. A. 1282
We describe a new variant of anion exchange chromatography in columns packed with porous particles that embody charged low-density polymer zones supported by a higher density polymer skeleton. IgG defies the norms of anion exchange and is excluded to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54d4bc60b02347cdd9c94d18fad9e64e
https://pubmed.ncbi.nlm.nih.gov/23422893
https://pubmed.ncbi.nlm.nih.gov/23422893
Autor:
Corrine Wan, Pete Gagnon, Do Yun Kim, Muriel Bardor, Monika Mueller, Kong Meng Hoi, Hui Theng Gan
Publikováno v:
Journal of Pharmaceutical Sciences
Journal of Pharmaceutical Sciences, Wiley, 2013, 102 (3), pp.1125-1132. ⟨10.1002/jps.23428⟩
Journal of Pharmaceutical Sciences, Wiley, 2013, 102 (3), pp.1125-1132. ⟨10.1002/jps.23428⟩
Tolerance of low pH is crucial for recombinant proteins to survive conditions that might be experienced during manufacturing such as virus inactivation and elution from bioaffinity columns. In this study, we exposed three different purified immunoglo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d8355edecd2d60374060c2422cc6b1f
https://pubmed.ncbi.nlm.nih.gov/23280704
https://pubmed.ncbi.nlm.nih.gov/23280704
Autor:
Thorsten Wohland, Tapan Kumar Mistri, Chen Sok Lam, L. Lim, Hui Theng Gan, Yong Hwee Foo, David Rodda, Thankiah Sudhaharan, Sohail Ahmed, Chai Chou, Paul Robson
Publikováno v:
The Biochemical journal. 448(1)
Oct4 and Sox2 are two essential transcription factors that co-regulate target genes for the maintenance of pluripotency. However, it is unclear whether they interact prior to DNA binding or how the target sites are accessed in the nucleus. By generat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7997e2b58bbd47f01966e9a31935f1bd
https://pubmed.ncbi.nlm.nih.gov/22909387
https://pubmed.ncbi.nlm.nih.gov/22909387