Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Autor: Pau Ferrer, Joan Albiol, Marc Carnicer, Hugo Cueto Rojas, A. Wahl, Joel Jordà

Publikováno v: Europe PubMed Central
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Metabolites, Vol 4, Iss 2, Pp 281-299 (2014)
Metabolites
Metabolites; Volume 4; Issue 2; Pages: 281-299
Metabolites, 4 (2), 2014

Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fd793a08b0ae2e35452944fa5cf5f4a
http://europepmc.org/abstract/med/24957027