Nanopore ReCappable sequencing maps SARS-CoV-2 5' capping sites and provides new insights into the structure of sgRNAs

Autor: Camilla Ugolini, Logan Mulroney, Adrien Leger, Matteo Castelli, Elena Criscuolo, Maia Kavanagh Williamson, Andrew D Davidson, Abdulaziz Almuqrin, Roberto Giambruno, Miten Jain, Gianmaria Frigè, Hugh Olsen, George Tzertzinis, Ira Schildkraut, Madalee G Wulf, Ivan R Corrêa, Laurence Ettwiller, Nicola Clementi, Massimo Clementi, Nicasio Mancini, Ewan Birney, Mark Akeson, Francesco Nicassio, David A Matthews, Tommaso Leonardi

Publikováno v: Ugolini, C, Mulroney, L, Leger, A, Castelli, M, Criscuolo, E, Kavanagh Williamson, M, Davidson, A D, Almuqrin, A M, Giambruno, R, Jain, M, Frige, G, Olsen, H, Tzertzinis, G, Schildkraut, I, Wulf, M G, Corrêa Jr, I R, Ettwiller, L, Clementi, N, Clementi, M, Mancini, N, Birney, E, Akeson, M, Nicassio, F, Matthews, D A & Leonardi, T 2022, ' Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs ', Nucleic Acids Research, vol. 50, no. 6, gkac144, pp. 3475-3489 . https://doi.org/10.1093/nar/gkac144

The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length tran

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https://pubmed.ncbi.nlm.nih.gov/35244721