Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Huey G. Lee"'
Autor:
Edward Fritsche, Huey G. Lee
Publikováno v:
Journal of Chromatography A. 994:213-219
Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) is performed to quantify monomeric avidin and biotin on a protein. Under non-reducing SDS-CGE conditions, avidin migrates as monomers exhibiting apparent molecular mass 17,000. In the pre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::debe07ab0ad4505fd2a8e6acbb91056d
https://doi.org/10.1016/s0021-9673(03)00440-0
https://doi.org/10.1016/s0021-9673(03)00440-0
Publikováno v:
Journal of Chromatography A. 947:143-149
Sodium dodecyl sulfate capillary gel electrophoresis has been used to separate and quantify murine monoclonal antibodies. The method uses a murine IgG, whose subclass differs from the analyte antibody, as an internal reference. The internal reference
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66cf1bdee4c4f8f0d55dea800d26538d
https://doi.org/10.1016/s0021-9673(01)01590-4
https://doi.org/10.1016/s0021-9673(01)01590-4
Autor:
Huey G Lee
Publikováno v:
Journal of Immunological Methods. 234:71-81
High-performance sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) has been used to separate antibodies and their fragments according to size. Under non-reduced conditions, murine monoclonal antibodies generally show a predominant peak w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::163e7450a8f3ad1007808b420f3ff3b6
https://doi.org/10.1016/s0022-1759(99)00205-7
https://doi.org/10.1016/s0022-1759(99)00205-7
Autor:
Huey G. Lee, Dominic M. Desiderio
Publikováno v:
Analytica Chimica Acta. 383:79-99
Capillary zone electrophoresis (CZE) is a liquid phase separation technique that can be used to purify and to analyze peptides in complex biological tissues or fluids. The application of CZE to opioid peptides is discussed in this review. The review
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0623f604d57246dc4406817ea4801f67
https://doi.org/10.1016/s0003-2670(98)00490-5
https://doi.org/10.1016/s0003-2670(98)00490-5
Autor:
Huey G Lee
Publikováno v:
Journal of Chromatography A. 790:215-223
Rapid (5 min) high-performance isoelectric focusing was performed in uncoated fused-silica capillaries to resolve isoforms of monoclonal antibodies and to determine their isoelectric points (pI). The methodology involved the use of a 32 cm (effective
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a1b8ce037430c63d938289cfd4c1bf4
https://doi.org/10.1016/s0021-9673(97)00749-8
https://doi.org/10.1016/s0021-9673(97)00749-8
Autor:
Dominic M. Desiderio, Huey G. Lee
Publikováno v:
Journal of Chromatography A. 686:309-317
Preparative capillary zone electrophoresis of three synthetic peptides was performed either manually or automatically by simple manipulations of a commercial electropherograph that is equipped only with an autosampler without any built-in fraction co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f87fa8ff47c817ebf7b81ec1427b01e7
https://doi.org/10.1016/0021-9673(94)00824-8
https://doi.org/10.1016/0021-9673(94)00824-8
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1163:309-314
We have reacted calmodulins containing cysteines substituted at positions 3 and 146 or 5 and 146 with bismaleimidohexane (BMH) to generate intramolecularly cross-linked proteins termed BMHCM or BMHCM1, respectively. Reactions were also performed with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74054e46de9cb63a913b5165ea8ae415
https://doi.org/10.1016/0167-4838(93)90167-p
https://doi.org/10.1016/0167-4838(93)90167-p
Autor:
Dominic M. Desiderio, Huey G. Lee
Publikováno v:
Journal of chromatography. B, Biomedical sciences and applications. 691(1)
Capillary zone electrophoresis (CZE) of five model proteins (lysozyme, myoglobin, ribonuclease A, alpha-lactalbumin, and trypsinogen), using ammonium formate as the electrophoretic buffer and triethylamine (TEA) as a buffer additive at pH 2.5, was us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dd777505418f2e47ffa99a14cdf956a
https://pubmed.ncbi.nlm.nih.gov/9140759
https://pubmed.ncbi.nlm.nih.gov/9140759
Publikováno v:
Analytical biochemistry. 229(2)
One proopiomelanocortin peptide [β-endorphin (BE)] and two proenkephalin A peptides [methionine enkephalin (ME) and leucine enkephalin (LE); LE derives also from proenkephalin B] were searched for in a bovine pituitary extract by capillary zone elec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d68cb13d67c177a91623205462c6d3f
https://pubmed.ncbi.nlm.nih.gov/7485971
https://pubmed.ncbi.nlm.nih.gov/7485971
Autor:
Jill Soble-Smith, James T. Robertson, Robert R. Becklin, George V. Huffmon, Huey G. Lee, Dominic M. Desiderio, Jih-Lie Tseng
Publikováno v:
Journal of chromatography. B, Biomedical applications. 667(2)
Capillary reversed-phase high-performance liquid chromatography (RP-HPLC) was used to determine acetylmethylprednisolone (A-MP) that had been administered to feline spinal cord tissue. The method used a 300 mm x 0.32 mm I.D. packed capillary octadecy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba4e1b114a23dd23b641866d3af2c55a
https://pubmed.ncbi.nlm.nih.gov/7663698
https://pubmed.ncbi.nlm.nih.gov/7663698