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Publikováno v:
Molecular and cellular probes. 51
The duplex real-time PCR assay based on SYBR Green І was developed for detection of porcine epidemic diarrhea virus (PEDV) and porcine bocavirus (PBoV) 3/4/5 genotypes simultaneously. Two pairs of specific primers were designed targeting the N gene
Autor:
Hui-Hua Zheng, Hao-Ying Han, Hua-Lin Hou, Ming-Fan Yang, Peng-Li Xu, Yu Zhao, Hong-Ying Chen, Run-Bo Tian
Publikováno v:
Molecular and Cellular Probes
The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay speci