Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Hua-Lei Liu"'
Autor:
Ji-Hui Jin, Jing-Jing Wang, Ying-Chao Ren, Shuo Liu, Jin-Ping Li, Guang-Yu Hou, Hua-Lei Liu, Qing-Ye Zhuang, Su-Chun Wang, Wen-Ming Jiang, Xiao-Hui Yu, Jian-Min Yu, Li-Ping Yuan, Cheng Peng, Guo-Zhong Zhang, Ji-Ming Chen
Publikováno v:
PeerJ, Vol 9, p e10748 (2021)
Background Avian paramyxoviruses (APMVs), also termed avian avulaviruses, are of a vast diversity and great significance in poultry. Detection of all known APMVs is challenging, and distribution of APMVs have not been well investigated. Methods A set
Externí odkaz:
https://doaj.org/article/778de67632784c2fac27c6169d49a925
[Detection of an NA gene molecular marker in H7N9 subtype avian influenza viruses by pyrosequencing]
Autor:
Yong-Gang, Zhao, Hua-Lei, Liu, Jing-Jing, Wang, Dong-Xia, Zheng, Yun-Ling, Zhao, Sheng-Qiang, Ge, Zhi-Liang, Wang
Publikováno v:
Bing du xue bao = Chinese journal of virology. 30(4)
This study aimed to establish a method for the detection and identification of H7N9 avian influenza viruses based on the NA gene by pyrosequencing. According to the published NA gene sequences of the avian influenza A (H7N9) virus, a 15-nt deletion w
Publikováno v:
Bing du xue bao = Chinese journal of virology. 28(5)
Based on the genomic sequence of NDV08-004 strain (GenBank accession number FJ794269), seven pairs of primers were designed to amplify the genomic fragments by RT-PCR and cloned into pGEM-Teasy vector. The fragments (named A to G) were sub-cloned int
Autor:
Hua-lei, Liu, Yan, Lv, Dong-xia, Zheng, Yun-ling, Zhao, Cheng-ying, Sun, Wei, Zhang, Jin-ming, Li, Zhi-liang, Wang
Publikováno v:
Bing du xue bao = Chinese journal of virology. 26(5)
Mutation in any of five key amino acid residues (at positions 26, 27, 30, 31 and 34) within the M2 protein of influenza A viruses leads to resistance against the amantodine class of anti-influenza drugs. In this study, a pyrosequencing method was des
Autor:
Hua-Lei, Liu, Wei, Zhang, Bei-Xia, Hu, Yun-Ling, Zhao, Yan-Yan, Huang, Dong-Xia, Zheng, Fei, Chen, Cheng-Ying, Sun, Yan-Gong, Wu, Xiu-Mei, Zhang, Zhi-Liang, Wang
Publikováno v:
Bing du xue bao = Chinese journal of virology. 25(5)
Thirteen isolates of Class I Newcastle disease virus obtained from healthy poultry in China during 2008 were characterized genotypically in this study. All the isolates were proved to be lentogenic strains based on the deduced amino acid sequence of
Publikováno v:
Wei sheng wu xue bao = Acta microbiologica Sinica. 45(4)
The site-directed mutagenesis of HA gene was made by using PCR, and mismatches were introduced into primers. Mutagenesis was performed in a three-step PCR. The amplified fragments from the second PCR which contained the mutation site were cloned into
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 21(1)
One highly pathogenic strain of avian influenza virus (AIV) was isolated from goose in China recently, designated as F-3. In order to study the viral entry mechanisms, the hemagglutinin (HA) gene of H5N1 subtype AIV isolate was amplified by RT-PCR, a
Publikováno v:
Wei sheng wu xue bao = Acta microbiologica Sinica. 45(1)
Using a pair of specific primers designed according to the relevant nucleotide sequence from GenBank, the HA1 gene of H5N1 subtype AIV was amplified with PCR method. The PCR product was cloned into pET-32a(+) to get a prokaryotic recombinant plasmid
Publikováno v:
Sheng li xue bao : [Acta physiologica Sinica]. 56(6)
The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded by using intracellular microelectrode technique. The results ob