Generation of a homozygous (MCRIi031-A-3) WT1 knockout human iPSC line.

Autor: Pachernegg S; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia., Robevska G; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia., Ferreira LGA; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Laboratory of Molecular and Translational Endocrinology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil., van den Bergen JA; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia., Vlahos K; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia., Howden SE; Department of Paediatrics, The University of Melbourne, Melbourne, Australia; iPSC Derivation and Gene Editing Facility, Murdoch Children's Research Institute, Melbourne, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children's Research Institute, Melbourne, Australia., Sinclair AH; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia., Ayers KL; Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia. Electronic address: katie.ayers@mcri.edu.au.

Publikováno v: Stem cell research [Stem Cell Res] 2024 Sep; Vol. 79, pp. 103494. Date of Electronic Publication: 2024 Jul 10.

A genetically inducible endothelial niche enables vascularization of human kidney organoids with multilineage maturation and emergence of renin expressing cells.

Autor: Maggiore JC; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., LeGraw R; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Przepiorski A; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Velazquez J; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Chaney C; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Vanichapol T; Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand., Streeter E; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Almuallim Z; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Oda A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Chiba T; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Silva-Barbosa A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Franks J; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Hislop J; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Hill A; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Wu H; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA., Pfister K; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Watkins SC; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Little MH; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia., Humphreys BD; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA; Department of Developmental Biology, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, USA., Kiani S; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Watson A; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Stolz DB; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Davidson AJ; Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand., Carroll T; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Cleaver O; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Sims-Lucas S; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh Pennsylvania, USA., Ebrahimkhani MR; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address: mo.ebr@pitt.edu., Hukriede NA; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; Center for Integrative Organ Systems, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. Electronic address: hukriede@pitt.edu.

Publikováno v: Kidney international [Kidney Int] 2024 Dec; Vol. 106 (6), pp. 1086-1100. Date of Electronic Publication: 2024 Jun 18.

Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing.

Autor: Houweling PJ; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia. Electronic address: Peter.houweling@mcri.edu.au., Crossman V; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Tiong CF; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Coles CA; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia., Taylor RL; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia., Clayton JS; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia., Graham A; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Vlahos K; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., North KN; Murdoch Children's Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia.

Publikováno v: Stem cell research [Stem Cell Res] 2024 Mar; Vol. 75, pp. 103313. Date of Electronic Publication: 2024 Jan 23.

Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.

Autor: Maggiore JC; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., LeGraw R; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Przepiorski A; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., Velazquez J; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Chaney C; Department of Molecular Biology and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Streeter E; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., Silva-Barbosa A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Franks J; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Hislop J; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA., Hill A; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Wu H; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130., Pfister K; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Watkins SC; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Little M; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.; Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia.; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia., Humphreys BD; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130.; Department of Developmental Biology, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130., Watson A; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Stolz DB; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Kiani S; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA., Davidson AJ; Department of Molecular Medicine and Pathology, University of Auckland, Auckland 1010, New Zealand., Carroll TJ; Department of Molecular Biology and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Cleaver O; Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390., Sims-Lucas S; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Ebrahimkhani MR; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA., Hukriede NA; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 May 30. Date of Electronic Publication: 2023 May 30.

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids.

Autor: Vanslambrouck JM; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia., Wilson SB; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia., Tan KS; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia., Groenewegen E; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia., Rudraraju R; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia., Neil J; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia., Lawlor KT; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia., Mah S; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia., Scurr M; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia., Howden SE; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia., Subbarao K; Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia., Little MH; Murdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia. melissa.little@mcri.edu.au.; Department of Paediatrics, The University of Melbourne, Parkville, VIC, Australia. melissa.little@mcri.edu.au.; Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, VIC, Australia. melissa.little@mcri.edu.au.

Publikováno v: Nature communications [Nat Commun] 2022 Oct 08; Vol. 13 (1), pp. 5943. Date of Electronic Publication: 2022 Oct 08.