PCR revisited: a case for revalidation of PCR assays for microorganisms using identification of Campylobacter species as an exemplar

Autor: On S.L.W.1, Brandt S.M. 1, Cornelius A.J. 1, Fusco V. 2, Quero G.M. 2, Ma?kiw E. 3, 4, Houf K. 5, Bilbao A. 6, Díaz A.I. 6, Benejat L. 7, Megraud F. 7, Collins-Emerson J. 8, French N.P. 8, Gotcheva V. 9, Angelov, A. 9, Alakomi, H.-L. 10, Saarela M. 10, Paulin S.M.* 1

Publikováno v: Quality assurance and safety of crops & foods
5 (2013): 49–62. doi:10.3920/QAS2012.0158
info:cnr-pdr/source/autori:On S.L.W.1, Brandt S.M. 1, Cornelius A.J. 1, Fusco V. 2, Quero G.M. 2, Ma?kiw E. 3, 4, Houf K. 5, Bilbao A. 6, Díaz A.I. 6, Benejat L. 7, Megraud F. 7, Collins-Emerson J. 8, French N.P. 8, Gotcheva V. 9, Angelov, A. 9, Alakomi, H.-L. 10, Saarela M. 10, Paulin S.M.* 1/titolo:PCR revisited: a case for revalidation of PCR assays for microorganisms using identification of Campylobacter species as an exemplar./doi:10.3920%2FQAS2012.0158/rivista:Quality assurance and safety of crops & foods (Print)/anno:2013/pagina_da:49/pagina_a:62/intervallo_pagine:49–62/volume:5
On, S L W, Brandt, S M, Cornelius, A J, Fusco, V, Quero, G M, Mackiw, E, Houf, K, Bilbao, A, Diaz, A I, Benejat, L, Megraud, F, Collins-Emerson, J, French, N P, Gotcheva, V, Angelov, A, Alakomi, H-L, Saarela, M & Paulin, S M 2013, ' PCR revisited : A case for revalidation of PCR assays for microorganisms using identification of Campylobacter species as an exemplar ', Quality Assurance and Safety of Crops and Foods, vol. 5, pp. 49-62 . https://doi.org/10.3920/QAS2012.0158

We re-examined the sensitivity and specificity of 31 PCR assays (including four commercially available and developed in-house methods) for the identification of Campylobacter species, with particular reference to taxa described since 2004, which are

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