Alternative Enzymes Lead to Improvements in Sequence Coverage and PTM Analysis

Autor: Hooper, Kyle, Rosenblatt, Michael, Urh, Marjeta, Saveliev, Sergei, Hosfield, Chris, Kobs, Gary, Ford, Michael, Jones, Richard, Amunugama, Ravi, Allen, David, Brazas, Robert

The profiling of proteins using biological mass spectrometry (bottom up proteomics) most commonly requires trypsin. Trypsin is advantageous in that it produces peptides of optimal charge and size. However, for applications in which the proteins under

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmc_________::08720fd8b2778ba34cb9a4b6e4c5ce4a
https://europepmc.org/articles/PMC3635366/

Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays.

Autor: Crummy EK; Promega Corporation, Madison, WI, United States. Electronic address: ellen.crummy@promega.com., Caine EA; Promega Corporation, Madison, WI, United States., Mikheil D; Promega Corporation, Madison, WI, United States., Corona C; Promega Biosciences Incorporated, San Luis Obispo, CA, United States., Riching KM; Promega Corporation, Madison, WI, United States., Hosfield C; Promega Corporation, Madison, WI, United States., Urh M; Promega Corporation, Madison, WI, United States.

Publikováno v: Methods in enzymology [Methods Enzymol] 2023; Vol. 681, pp. 81-113. Date of Electronic Publication: 2022 Dec 12.