Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Horacio G. Nastri"'
Autor:
Andre A. R. Teixeira, Sara D’Angelo, M. Frank Erasmus, Camila Leal-Lopes, Fortunato Ferrara, Laura P. Spector, Leslie Naranjo, Esteban Molina, Tamara Max, Ashley DeAguero, Katherine Perea, Shaun Stewart, Rebecca A. Buonpane, Horacio G. Nastri, Andrew R. M. Bradbury
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
Affinity maturation is often a necessary step for the development of potent therapeutic molecules. Many different diversification strategies have been used for antibody affinity maturation, including error-prone PCR, chain shuffling, and targeted com
Externí odkaz:
https://doaj.org/article/33ec78a25c5c442aa8059bd64ee24afc
Autor:
M. Frank Erasmus, Sara D’Angelo, Fortunato Ferrara, Leslie Naranjo, André A. Teixeira, Rebecca Buonpane, Shaun M. Stewart, Horacio G. Nastri, Andrew R. M. Bradbury
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-16 (2021)
Here, the authors construct and validate an in vitro antibody library from a single, healthy donor. They demonstrate the functionality of the library using several therapeutically relevant targets and were able to obtain many different antibodies wit
Externí odkaz:
https://doaj.org/article/97589959248d40069c6d04e7cbd5a7d0
Autor:
Sara D'Angelo, André Azevedo Reis Teixeira, Leslie Naranjo, Andrew Bradbury, Fortunato Ferrara, M. Frank Erasmus, Shaun M. Stewart, Horacio G. Nastri, Rebecca Buonpane
Publikováno v:
Communications Biology
Communications Biology, Vol 4, Iss 1, Pp 1-16 (2021)
Communications Biology, Vol 4, Iss 1, Pp 1-16 (2021)
Antibody complementarity determining region diversity has been considered to be the most important metric for the production of a functional antibody library. Generally, the greater the antibody library diversity, the greater the probability of selec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09849ee0b18e2827a2dd17b0eb480413
https://doi.org/10.1038/s42003-021-01881-0
https://doi.org/10.1038/s42003-021-01881-0
Publikováno v:
Journal of Molecular Biology. 284:1491-1504
The PvuII restriction endonuclease is a homodimer that recognizes and cleaves the DNA sequence 5′-CAGCTG-3′ in double-stranded DNA, and the structure of this enzyme has been reported. In the wild-type enzyme, Asp34 interacts with the internal gua
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f494949e527a25eaf51d443163d73a80
https://doi.org/10.1006/jmbi.1998.2269
https://doi.org/10.1006/jmbi.1998.2269
Autor:
Horacio G. Nastri, Kendall L. Knight
Publikováno v:
Journal of Biological Chemistry. 269:26311-26322
Using a combinatorial cassette mutagenesis procedure we have introduced a large number of single and multiple amino acid substitutions into an area of the RecA protein defined by residues 152-159. This sequence overlaps the disordered loop 1 region (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b5d0f2983ff0846c6e38ab278d0c97eb
https://doi.org/10.1016/s0021-9258(18)47195-5
https://doi.org/10.1016/s0021-9258(18)47195-5
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1216:455-459
The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d05bfeb8abe5e674e28e671ec108134
https://doi.org/10.1016/0167-4781(93)90014-5
https://doi.org/10.1016/0167-4781(93)90014-5
Publikováno v:
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 12(4)
The hydrolysis of phosphodiester bonds by nucleases is critical to nucleic acid processing. Many nucleases utilize metal ion cofactors, and for a number of these enzymes two active-site metal ions have been detected. Testing proposed mechanistic role
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a2f7e6c88064e3bb0ae28c86cf00810
https://pubmed.ncbi.nlm.nih.gov/17308914
https://pubmed.ncbi.nlm.nih.gov/17308914
Publikováno v:
The Journal of biological chemistry. 272(41)
The role of particular residues of the PvuII endonuclease in DNA binding and cleavage was studied by mutational analysis using a number of in vivo and in vitro approaches. While confirming the importance of residues predicted to be involved directly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cf20c32c304d1cded2007ea18c6eb4e
https://pubmed.ncbi.nlm.nih.gov/9325303
https://pubmed.ncbi.nlm.nih.gov/9325303
Publikováno v:
The Journal of biological chemistry. 270(15)
The functional significance of residues in the RecA protein P-loop motif was assessed by analyzing 100 unique mutants with single amino acid substitutions in this region. Comparison of the effects on the LexA coprotease and recombination activities s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c75dbf92e05bc0c65cde10932b6e01d
https://pubmed.ncbi.nlm.nih.gov/7721735
https://pubmed.ncbi.nlm.nih.gov/7721735
Autor:
Horacio G. Nastri, I.D. Algranati
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 949:65-70
Amino-acid starvation in polyamine-auxotrophic bacteria grown in the presence of putrescine provokes a marked inhibition of protein synthesis. This inhibition is almost completely relieved in polyamine-depleted cells. The differential behaviour of ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61ec00c5dd6c8c6131e3a9def658a31e
https://doi.org/10.1016/0167-4781(88)90055-3
https://doi.org/10.1016/0167-4781(88)90055-3