An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Autor: Schoof, Michael, Faust, Bryan, Saunders, Reuben A, Sangwan, Smriti, Rezelj, Veronica, Hoppe, Nick, Boone, Morgane, Billesbølle, Christian B, Puchades, Cristina, Azumaya, Caleigh M, Kratochvil, Huong T, Zimanyi, Marcell, Deshpande, Ishan, Liang, Jiahao, Dickinson, Sasha, Nguyen, Henry C, Chio, Cynthia M, Merz, Gregory E, Thompson, Michael C, Diwanji, Devan, Schaefer, Kaitlin, Anand, Aditya A, Dobzinski, Niv, Zha, Beth Shoshana, Simoneau, Camille R, Leon, Kristoffer, White, Kris M, Chio, Un Seng, Gupta, Meghna, Jin, Mingliang, Li, Fei, Liu, Yanxin, Zhang, Kaihua, Bulkley, David, Sun, Ming, Smith, Amber M, Rizo, Alexandrea N, Moss, Frank, Brilot, Axel F, Pourmal, Sergei, Trenker, Raphael, Pospiech, Thomas, Gupta, Sayan, Barsi-Rhyne, Benjamin, Belyy, Vladislav, Barile-Hill, Andrew W, Nock, Silke, Liu, Yuwei, Krogan, Nevan J, Ralston, Corie Y, Swaney, Danielle L, García-Sastre, Adolfo, Ott, Melanie, Vignuzzi, Marco, QCRG Structural Biology Consortium, Walter, Peter, Manglik, Aashish

Publikováno v: Science (New York, N.Y.), vol 370, iss 6523

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus enters host cells via an interaction between its Spike protein and the host cell receptor angiotensin-converting enzyme 2 (ACE2). By screening a yeast surface-displayed library of

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An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike.

Autor: Schoof M; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA. michael@walterlab.ucsf.edu peter@walterlab.ucsf.edu aashish.manglik@ucsf.edu.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Faust B; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA.; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Saunders RA; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA., Sangwan S; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Rezelj V; Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Institut Pasteur, 75724 Paris Cedex 15, France., Hoppe N; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Boone M; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Billesbølle CB; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Puchades C; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Azumaya CM; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Kratochvil HT; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Zimanyi M; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Deshpande I; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Liang J; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA., Dickinson S; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Nguyen HC; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Chio CM; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Merz GE; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Thompson MC; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Diwanji D; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Schaefer K; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Anand AA; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Dobzinski N; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Zha BS; Department of Pulmonary, Critical Care, Allergy and Sleep Medicine, University of California San Francisco, San Francisco, CA, USA., Simoneau CR; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; J. David Gladstone Institutes, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Leon K; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; J. David Gladstone Institutes, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., White KM; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Chio US; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Gupta M; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Jin M; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Li F; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Liu Y; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Zhang K; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Bulkley D; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Sun M; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Smith AM; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Rizo AN; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Moss F; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Brilot AF; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Pourmal S; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Trenker R; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Pospiech T; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA., Gupta S; Molecular Biophysics and Integrated Bioimaging and the Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Barsi-Rhyne B; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA., Belyy V; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Barile-Hill AW; Cytiva Life Sciences, Marlborough, MA, USA., Nock S; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Liu Y; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Krogan NJ; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA.; Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; J. David Gladstone Institutes, San Francisco, CA, USA., Ralston CY; Molecular Biophysics and Integrated Bioimaging and the Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Swaney DL; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA.; Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; J. David Gladstone Institutes, San Francisco, CA, USA., García-Sastre A; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Ott M; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; J. David Gladstone Institutes, San Francisco, CA, USA.; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Vignuzzi M; Viral Populations and Pathogenesis Unit, CNRS UMR 3569, Institut Pasteur, 75724 Paris Cedex 15, France., Walter P; Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA. michael@walterlab.ucsf.edu peter@walterlab.ucsf.edu aashish.manglik@ucsf.edu.; Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA., Manglik A; Department of Pharmaceutical Chemistry, University of California at San Francisco, San Francisco, CA, USA. michael@walterlab.ucsf.edu peter@walterlab.ucsf.edu aashish.manglik@ucsf.edu.; Quantitative Biosciences Institute (QBI) Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA, USA.; Quantitative Biosciences Institute (QBI), University of California San Francisco, San Francisco, CA, USA.; Department of Anesthesia and Perioperative Care, University of California at San Francisco, San Francisco, CA, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2020 Dec 18; Vol. 370 (6523), pp. 1473-1479. Date of Electronic Publication: 2020 Nov 05.