Modulating Protein-Protein Interactions of the Mitotic Polo-like Kinases to Target Mutant KRAS

Autor: Narvaez, AJ, Ber, S, Crooks, A, Emery, A, Hardwick, B, Guarino Almeida, E, Huggins, DJ, Perera, D, Roberts-Thomson, M, Azzarelli, R, Hood, FE, Prior, IA, Walker, DW, Boyce, R, Boyle, RG, Barker, SP, Torrance, CJ, McKenzie, GJ, Venkitaraman, AR

Mutations activating KRAS underlie many forms of cancer, but are refractory to therapeutic targeting. Here, we develop Poloppin, an inhibitor of protein-protein interactions via the Polo-box domain (PBD) of the mitotic Polo-like kinases (PLKs), in mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::beb31fa33546d5692971bfee36b6f8e2

Absolute Quantitation of GTPase Protein Abundance.

Autor: Hood FE; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK., Sahraoui YM; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK., Jenkins RE; Centre for Drug Safety Science Bioanalytical Facility, Division of Molecular and Clinical Pharmacology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK., Prior I; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK. iprior@liv.ac.uk.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2262, pp. 65-90.

Isoform-specific Ras signaling is growth factor dependent.

Autor: Hood FE; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom., Klinger B; Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.; Integrative Research Institute for the Life Sciences, Humboldt-Universität zu Berlin, 10099 Berlin, Germany.; Institute for Theoretical Biology, Humboldt-Universität zu Berlin, 10115 Berlin, Germany., Newlaczyl AU; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom., Sieber A; Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.; Integrative Research Institute for the Life Sciences, Humboldt-Universität zu Berlin, 10099 Berlin, Germany.; Institute for Theoretical Biology, Humboldt-Universität zu Berlin, 10115 Berlin, Germany., Dorel M; Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.; Integrative Research Institute for the Life Sciences, Humboldt-Universität zu Berlin, 10099 Berlin, Germany.; Institute for Theoretical Biology, Humboldt-Universität zu Berlin, 10115 Berlin, Germany., Oliver SP; Department of Biological Sciences, University of Chester, CH1 4BJ Chester, United Kingdom., Coulson JM; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom., Blüthgen N; Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.; Integrative Research Institute for the Life Sciences, Humboldt-Universität zu Berlin, 10099 Berlin, Germany.; Institute for Theoretical Biology, Humboldt-Universität zu Berlin, 10115 Berlin, Germany., Prior IA; Division of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom.

Publikováno v: Molecular biology of the cell [Mol Biol Cell] 2019 Apr 15; Vol. 30 (9), pp. 1108-1117. Date of Electronic Publication: 2019 Feb 20.

Modulating Protein-Protein Interactions of the Mitotic Polo-like Kinases to Target Mutant KRAS.

Autor: Narvaez AJ; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Ber S; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Crooks A; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Emery A; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Hardwick B; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Guarino Almeida E; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Huggins DJ; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK; University of Cambridge, Theory of Condensed Matter Group, Cavendish Laboratory, 19 J J Thomson Avenue, Cambridge CB3 0HE, UK., Perera D; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Roberts-Thomson M; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK., Azzarelli R; Department of Oncology, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK; Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK., Hood FE; Division of Cellular and Molecular Physiology, Crown Street, University of Liverpool, Liverpool L69 3BX, UK., Prior IA; Division of Cellular and Molecular Physiology, Crown Street, University of Liverpool, Liverpool L69 3BX, UK., Walker DW; Sentinel Oncology Ltd., Cambridge Science Park, Milton Road, Cambridge CB4 0EY, UK., Boyce R; Sentinel Oncology Ltd., Cambridge Science Park, Milton Road, Cambridge CB4 0EY, UK., Boyle RG; Sentinel Oncology Ltd., Cambridge Science Park, Milton Road, Cambridge CB4 0EY, UK., Barker SP; PhoreMost Ltd., Babraham Research Campus, Cambridge CB22 3AT, UK., Torrance CJ; PhoreMost Ltd., Babraham Research Campus, Cambridge CB22 3AT, UK., McKenzie GJ; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK; PhoreMost Ltd., Babraham Research Campus, Cambridge CB22 3AT, UK., Venkitaraman AR; Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK. Electronic address: arv22@mrc-cu.cam.ac.uk.

Publikováno v: Cell chemical biology [Cell Chem Biol] 2017 Aug 17; Vol. 24 (8), pp. 1017-1028.e7. Date of Electronic Publication: 2017 Aug 10.

The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.

Autor: Nixon FM; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom., Gutiérrez-Caballero C; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom., Hood FE; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Booth DG; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Prior IA; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom., Royle SJ; Division of Biomedical Cell Biology, Warwick Medical School, Coventry, United Kingdom.

Publikováno v: ELife [Elife] 2015 Jun 19; Vol. 4. Date of Electronic Publication: 2015 Jun 19.