Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

Autor: Leonie Asfora Sarubbo, Ana Lúcia Figueiredo Porto, Keila Aparecida Moreira, José Luis Lima-Filho, Homero José Farias de Melo, Elias Basile Tambourgi, Galba Maria de Campos-Takaki

Publikováno v: Brazilian Archives of Biology and Technology, Vol 47, Iss 5, Pp 821-826 (2004)
Brazilian Archives of Biology and Technology v.47 n.5 2004
Brazilian Archives of Biology and Technology
Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
Brazilian Archives of Biology and Technology, Volume: 47, Issue: 5, Pages: 821-826, Published: SEP 2004

A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019