Homology-Directed Recombination for Enhanced Engineering of Chimeric Antigen Receptor T Cells

Autor: Malika Hale, Baeckseung Lee, Yuchi Honaker, Wai-Hang Leung, Alexandra E. Grier, Holly M. Jacobs, Karen Sommer, Jaya Sahni, Shaun W. Jackson, Andrew M. Scharenberg, Alexander Astrakhan, David J. Rawlings

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 4, Iss C, Pp 192-203 (2017)

Gene editing by homology-directed recombination (HDR) can be used to couple delivery of a therapeutic gene cassette with targeted genomic modifications to generate engineered human T cells with clinically useful profiles. Here, we explore the functio

Externí odkaz: https://doaj.org/article/5c8a965f6c324b0c809e18b4b234bc06

The Sia System and c-di-GMP Play a Crucial Role in Controlling Cell-Association of Psl in Planktonic P. aeruginosa

Autor: Julia E. Dreifus, Lindsey O’Neal, Holly M. Jacobs, Adithya S. Subramanian, P. Lynne Howell, Daniel J. Wozniak, Matthew R. Parsek

Publikováno v: J Bacteriol

Many bacterial species use the secondary messenger, c-di-GMP, to promote the production of biofilm matrix components. In Pseudomonas aeruginosa, c-di-GMP production is stimulated upon initial surface contact and generally remains high throughout biof

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::024af546b9bbb42c28d6d11baa75d468
https://pubmed.ncbi.nlm.nih.gov/36448788

Mucoid Pseudomonas aeruginosa Can Produce Calcium-Gelled Biofilms Independent of the Matrix Components Psl and CdrA

Autor: Holly M. Jacobs, Lindsey O’Neal, Edward Lopatto, Daniel J. Wozniak, Thomas Bjarnsholt, Matthew R. Parsek

Publikováno v: Jacobs, H M, O'Neal, L, Lopatto, E, Wozniak, D J, Bjarnsholt, T & Parsek, M R 2022, ' Mucoid Pseudomonas aeruginosa Can Produce Calcium-Gelled Biofilms Independent of the Matrix Components Psl and CdrA ', Journal of Bacteriology, vol. 204, no. 5 . https://doi.org/10.1128/jb.00568-21

Biofilms are aggregates of microorganisms embedded in an extracellular matrix comprised largely of exopolysaccharides (EPSs), nucleic acids, and proteins. Pseudomonas aeruginosa is an opportunistic human pathogen that is also a model organism for stu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12c4eb51e213eb800c6c866e170e5482
https://pubmed.ncbi.nlm.nih.gov/35416688

The Pseudomonas aeruginosa homeostasis enzyme AlgL clears the periplasmic space of accumulated alginate during polymer biosynthesis

Autor: Andreea A. Gheorghita, Francis Wolfram, Gregory B. Whitfield, Holly M. Jacobs, Roland Pfoh, Steven S.Y. Wong, Allison K. Guitor, Mara C. Goodyear, Alison M. Berezuk, Cezar M. Khursigara, Matthew R. Parsek, P. Lynne Howell

Publikováno v: The Journal of biological chemistry. 298(2)

Pseudomonas aeruginosa is an opportunistic human pathogen and a leading cause of chronic infection in the lungs of individuals with cystic fibrosis. After colonization, P. aeruginosa often undergoes a phenotypic conversion to mucoidy, characterized b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2c5380d3886d07dd87391861139addc
https://pubmed.ncbi.nlm.nih.gov/34990713

The Versatile Pseudomonas aeruginosa Biofilm Matrix Protein CdrA Promotes Aggregation through Different Extracellular Exopolysaccharide Interactions

Autor: Michael Matwichuk, Matthew R. Parsek, Cynthis Wong, Courtney Reichhardt, Daniel J. Wozniak, Holly M. Jacobs

Publikováno v: Journal of Bacteriology

Depending upon the strain, Pseudomonas aeruginosa can use different exopolysaccharides (e.g., Psl, Pel, and alginate) to build its biofilm matrix. Previously, we demonstrated that the biofilm matrix protein CdrA binds to Psl, promoting biofilm format

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21aa9bb61b71c93e1f135b29ed813a65
https://pubmed.ncbi.nlm.nih.gov/32661078