Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Holly C. Gibbs"'
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102040- (2023)
Summary: A reproducible imaging protocol should include four main detailed sections. The first should describe the sample preparation and include details about the tissue and/or cell culture preparation, the staining procedure, the optical grade of t
Externí odkaz:
https://doaj.org/article/4c39286d5ad8415bbe24c883e603c165
Autor:
Hao Yu, Qiang Li, Alfredo Sandoval, Jr., Holly C. Gibbs, Amber English, Tiffany Dunn, John Moth, Hajira Elahi, Bo Chen
Publikováno v:
STAR Protocols, Vol 3, Iss 4, Pp 101759- (2022)
Summary: Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal
Externí odkaz:
https://doaj.org/article/136a035447f9461c89fbd00020d4325f
Autor:
Holly C. Gibbs, Sakina M. Mota, Nathan A. Hart, Sun Won Min, Alex O. Vernino, Anna L. Pritchard, Anindito Sen, Stan Vitha, Sreeja Sarasamma, Avery L. McIntosh, Alvin T. Yeh, Arne C. Lekven, Dylan A. McCreedy, Kristen C. Maitland, Lisa M. Perez
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 9 (2021)
From the combined perspective of biologists, microscope instrumentation developers, imaging core facility scientists, and high performance computing experts, we discuss the challenges faced when selecting imaging and analysis tools in the field of li
Externí odkaz:
https://doaj.org/article/7b9c40c88b904acc819188e096001d6f
Publikováno v:
Biophysical Reports, Vol 1, Iss 1, Pp 100003- (2021)
We introduce a computational approach to build three-dimensional (3D) surface mesh models of the early-stage zebrafish brain primordia from time-series microscopy images. The complexity of the early-stage brain primordia and lack of recognizable land
Externí odkaz:
https://doaj.org/article/f07c57a794fc478f8b10b404cd3b5db9
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
In plants, the trafficking mechanisms by which sterols move through the plant and into target cells are unknown. Earlier studies identified endosomes as primary candidates for internalization of sterols in plants, but these results have come into que
Externí odkaz:
https://doaj.org/article/2ea56a41bb414632bb82844f1b880a00
Autor:
John W. Seawright, Harini Sreenivasappa, Holly C. Gibbs, Samuel Padgham, Song Y. Shin, Christine Chaponnier, Alvin T. Yeh, Jerome P. Trzeciakowski, Christopher R. Woodman, Andreea Trache
Publikováno v:
Frontiers in Physiology, Vol 9 (2018)
Aging induces a progressive decline in vasoconstrictor responses in central and peripheral arteries. This study investigated the hypothesis that vascular smooth muscle (VSM) contractile function declines with age in soleus muscle feed arteries (SFA).
Externí odkaz:
https://doaj.org/article/99e70e9dbc494fe4b0d756a1750f091c
Publikováno v:
Frontiers in Neuroanatomy, Vol 11 (2017)
A constriction in the neural tube at the junction of the midbrain and hindbrain is a conserved feature of vertebrate embryos. The constriction is a defining feature of the midbrain-hindbrain boundary (MHB), a signaling center that patterns the adjace
Externí odkaz:
https://doaj.org/article/da81ab6a1b3e4a7bab1874ccdf991d26
Autor:
Oscar R. Benavides, Holly C. Gibbs, Berkley P. White, Roland Kaunas, Carl A. Gregory, Kristen C. Maitland
The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to meet this challenge. The use of spherical microcarriers, however, pre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0a59f89ef712bbf07808faa58c8cf97
https://doi.org/10.1101/2022.09.19.508168
https://doi.org/10.1101/2022.09.19.508168
Autor:
Oscar R. Benavides, Holly C. Gibbs, Berkley P. White, Roland Kaunas, Carl A. Gregory, Alex J. Walsh, Kristen C. Maitland
Publikováno v:
PLOS ONE. 18:e0282298
The adoption of cell-based therapies into the clinic will require tremendous large-scale expansion to satisfy future demand, and bioreactor-microcarrier cultures are best suited to meet this challenge. The use of spherical microcarriers, however, pre
Autor:
Holly C, Gibbs, Sreeja, Sarasamma, Oscar R, Benavides, David G, Green, Nathan A, Hart, Alvin T, Yeh, Kristen C, Maitland, Arne C, Lekven
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2440
Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently p