Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Holly A. Rees"'
Autor:
Yi Yu, Thomas C. Leete, David A. Born, Lauren Young, Luis A. Barrera, Seung-Joo Lee, Holly A. Rees, Giuseppe Ciaramella, Nicole M. Gaudelli
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.
Externí odkaz:
https://doaj.org/article/ab50974242654c18950c59b5e285c447
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of per
Externí odkaz:
https://doaj.org/article/be483dddb5c54b75a6dd4f6fc308c2d2
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Base editing allows the precise introduction of point mutations into cellular DNA without requiring double-stranded DNA breaks or homology-directed repair, which is inefficient in postmitotic cells. Here the authors demonstrate in vivo base editing o
Externí odkaz:
https://doaj.org/article/425b7eebb92745d5aeb9260de20796e0
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence
Externí odkaz:
https://doaj.org/article/08222d2b844046fe8d313002c7e42c08
Autor:
Holly A. Rees, Alexis C. Komor, Wei-Hsi Yeh, Joana Caetano-Lopes, Matthew Warman, Albert S. B. Edge, David R. Liu
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and
Externí odkaz:
https://doaj.org/article/b60c28c60af84bb2b31538ea1a91ab66
Publikováno v:
Molecular therapy : the journal of the American Society of Gene Therapy, vol 29, iss 11
Mol Ther
Mol Ther
The development of CRISPR-derived genome editing technologies has enabled the precise manipulation of DNA sequences within the human genome. In this review, we discuss the initial development and cellular mechanism of action of CRISPR nucleases and D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb85bb0d9c00614a1cc660b322626041
https://doi.org/10.1016/j.ymthe.2021.09.027
https://doi.org/10.1016/j.ymthe.2021.09.027
Autor:
Alexander Liquori, Ian Slaymaker, Daisy Lam, Giuseppe Ciaramella, Dieter Lam, Adam J. Hartigan, David A. Born, Jeremy Decker, Fei Ann Ran, S. Haihua Chu, Lo-I Cheng, Holly A. Rees, Jeffrey Marshall, Michael S. Packer, Bob Gantzer, Nicole M. Gaudelli, Jenny Olins, Luis A. Barrera, Yi Yu
Publikováno v:
The CRISPR Journal. 4:169-177
Base editors are fusions of a deaminase and CRISPR-Cas ribonucleoprotein that allow programmable installment of transition mutations without double-strand DNA break intermediates. The breadth of potential base editing targets is frequently limited by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2a2df0d40b5dcfb8fde74d08414d7f2
https://doi.org/10.1089/crispr.2020.0144
https://doi.org/10.1089/crispr.2020.0144
Autor:
Jonathan Yen, Giuseppe Ciaramella, Luis A. Barrera, Aaron Edwards, Alexander Liquori, Nicole M. Gaudelli, Dieter K. Lam, Holly A. Rees, Noris M. Solá-Esteves, Lauren Young, Jason Michael Gehrke, Conrad Rinaldi, Michael S. Packer, Seung-Joo Lee, Ian Slaymaker, Ryan Murray, David A. Born
Publikováno v:
Nature Biotechnology. 38:892-900
The foundational adenine base editors (for example, ABE7.10) enable programmable A•T to G•C point mutations but editing efficiencies can be low at challenging loci in primary human cells. Here we further evolve ABE7.10 using a library of adenosin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4866b5ba658a300870d7c3056e7b2469
https://doi.org/10.1038/s41587-020-0491-6
https://doi.org/10.1038/s41587-020-0491-6
Autor:
Max W. Shen, Mandana Arbab, Peyton B. Randolph, Tony P. Huang, Gregory A. Newby, Zaneta Matuszek, Tina Wang, Holly A. Rees, David R. Liu, Shannon M. Miller
Publikováno v:
Nature biotechnology
The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing Gs. Here, we report the evolution of three new SpCas9 variants that collectively re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4854f8fda8cc240d332cdf315fc1e91
https://doi.org/10.1038/s41587-020-0412-8
https://doi.org/10.1038/s41587-020-0412-8
Autor:
Seung-Joo Lee, David A. Born, Holly A. Rees, Giuseppe Ciaramella, Thomas Leete, Nicole M. Gaudelli, Lauren Young, Yi Yu, Luis A. Barrera
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Cytosine base editors (CBEs) enable efficient, programmable reversion of T•A to C•G point mutations in the human genome. Recently, cytosine base editors with rAPOBEC1 were reported to induce unguided cytosine deamination in genomic DNA and cellul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::067f3ed91e52d094d4b5d41d2b647dd0
http://europepmc.org/articles/PMC7189382
http://europepmc.org/articles/PMC7189382