hACE2-Induced Allosteric Activation in SARS-CoV versus SARS-CoV-2 Spike Assemblies Revealed by Structural Dynamics.

Autor: Chen C; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA.; Biological Physics Structure and Design Program, University of Washington, Seattle, Washington 98195, USA., Zhu R; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA., Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA., Díaz-Salinas MA; Department of Microbiology and Physiological Systems, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605, USA., Nguyen A; Biological Physics Structure and Design Program, University of Washington, Seattle, Washington 98195, USA., Munro JB; Department of Microbiology and Physiological Systems, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605, USA., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, USA.; Biological Physics Structure and Design Program, University of Washington, Seattle, Washington 98195, USA.

Publikováno v: ACS infectious diseases [ACS Infect Dis] 2023 Jun 09; Vol. 9 (6), pp. 1180-1189. Date of Electronic Publication: 2023 May 11.

Structural dynamics reveal isolate-specific differences at neutralization epitopes on HIV Env.

Autor: Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Naika GS; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Kephart SM; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Nguyen A; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.; Biological Physics, Structure and Design Graduate Program, University of Washington, Seattle, WA 98195, USA., Zhu R; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Benhaim MA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Guo W; Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA., Moore JP; Division of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA., Hu SL; Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA., Sanders RW; Division of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA.; Department of Medical Microbiology, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, the Netherlands., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.; Biological Physics, Structure and Design Graduate Program, University of Washington, Seattle, WA 98195, USA.

Publikováno v: IScience [iScience] 2022 May 23; Vol. 25 (6), pp. 104449. Date of Electronic Publication: 2022 May 23 (Print Publication: 2022).

Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice.

Autor: Mangala Prasad V; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Leaman DP; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA., Lovendahl KN; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Croft JT; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Benhaim MA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Zwick MB; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: zwick@scripps.edu., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA; Biological Physics, Structure and Design Graduate Program, University of Washington, Seattle, WA 98195, USA; Department of Microbiology, University of Washington, Seattle, WA 98195, USA. Electronic address: kklee@uw.edu.

Publikováno v: Cell [Cell] 2022 Feb 17; Vol. 185 (4), pp. 641-653.e17. Date of Electronic Publication: 2022 Feb 04.

Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2.

Autor: Walls AC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Fiala B; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Wrenn S; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Pham MN; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Murphy M; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Tse LV; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Shehata L; Department of Immunology, University of Washington, Seattle, WA 98109, USA., O'Connor MA; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Washington National Primate Research Center, Seattle, WA 98121, USA., Chen C; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA; Biological Physics Structure and Design Program, University of Washington, Seattle, WA 91895, USA., Navarro MJ; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Miranda MC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Pettie D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Ravichandran R; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Kraft JC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Ogohara C; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Palser A; Kymab Ltd., Babraham Research Campus, Cambridge, UK., Chalk S; Kymab Ltd., Babraham Research Campus, Cambridge, UK., Lee EC; Kymab Ltd., Babraham Research Campus, Cambridge, UK., Guerriero K; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Washington National Primate Research Center, Seattle, WA 98121, USA., Kepl E; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Chow CM; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Sydeman C; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Brown B; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Washington National Primate Research Center, Seattle, WA 98121, USA., Fuller JT; Department of Microbiology, University of Washington, Seattle, WA 98109, USA., Dinnon KH 3rd; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Gralinski LE; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Leist SR; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Gully KL; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Lewis TB; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Washington National Primate Research Center, Seattle, WA 98121, USA., Guttman M; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Chu HY; Department of Medicine, University of Washington, Seattle, WA 98109, USA., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA; Biological Physics Structure and Design Program, University of Washington, Seattle, WA 91895, USA., Fuller DH; Department of Microbiology, University of Washington, Seattle, WA 98109, USA; Washington National Primate Research Center, Seattle, WA 98121, USA; Center for Innate Immunity and Immune Disease, University of Washington, Seattle, WA 98109, USA., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Kellam P; Kymab Ltd., Babraham Research Campus, Cambridge, UK; Department of Infectious Disease, Imperial College, London, UK., Carter L; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Pepper M; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Sheahan TP; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. Electronic address: dveesler@uw.edu., King NP; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA. Electronic address: neil@ipd.uw.edu.

Publikováno v: Cell [Cell] 2020 Nov 25; Vol. 183 (5), pp. 1367-1382.e17. Date of Electronic Publication: 2020 Oct 31.

De novo design of tunable, pH-driven conformational changes.

Autor: Boyken SE; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Benhaim MA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Busch F; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA., Jia M; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA., Bick MJ; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Choi H; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA 20147, USA., Klima JC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Chen Z; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Graduate Program in Biological Physics, Structure, and Design, University of Washington, Seattle, WA, USA., Walkey C; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Mileant A; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.; Graduate Program in Biological Physics, Structure, and Design, University of Washington, Seattle, WA, USA., Sahasrabuddhe A; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA., Wei KY; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Bioengineering, University of California, Berkeley, CA 94720, USA., Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Byron S; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Quijano-Rubio A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Bioengineering, University of Washington, Seattle, WA 98195, USA., Sankaran B; Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720., King NP; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Lippincott-Schwartz J; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA 20147, USA., Wysocki VH; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.; Graduate Program in Biological Physics, Structure, and Design, University of Washington, Seattle, WA, USA., Baker D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. dabaker@uw.edu.; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2019 May 17; Vol. 364 (6441), pp. 658-664.