Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Hoda Aboleneen"'
Publikováno v:
BioTechniques, Vol 28, Iss 5, Pp 890-895 (2000)
E. coli expressing soluble recombinant HIV antigens were analyzed directly by MALDI-TOF mass spectrometry (MS) from bacterial colonies picked from agar plates. An HIV envelope (ENV) antigen construct, penvA, was expressed in E. coli by transformation
Externí odkaz:
https://doaj.org/article/14b1d782a2c940b482823e38069fe386
Publikováno v:
BioTechniques. 28:890-895
E. coli expressing soluble recombinant HIV antigens were analyzed directly by MALDI-TOF mass spectrometry (MS) from bacterial colonies picked from agar plates. An HIV envelope (ENV) antigen construct, penvA, was expressed in E. coli by transformation
Publikováno v:
Analytical Chemistry. 71:664A-667A
MALDI-TOFMS makes it possible to obtain timely, accurate information on the identity of the reagents produced for immunoassays
Publikováno v:
Therapeutic Drug Monitoring. 20:294-300
Therapeutic monitoring of the immunosuppressant cyclosporin A (CsA) is routinely performed by immunoassays to make individual dosage adjustments for patients after organ transplantation. High-performance liquid chromatography with ultraviolet detecti
Publikováno v:
Clinical Chemistry. 48:364-366
Estradiol measurements are important in evaluations of ovarian function, infertility, and menopause (1). The assays are challenging because physiologic concentrations of estradiol are typically
Publikováno v:
Clinical chemistry. 48(2)
Autor:
Hoda Aboleneen, Huaiqin Wu
Publikováno v:
Analytical biochemistry. 290(2)
The combination of exonuclease digestion and mass spectrometry has been widely used for sequencing oligonucleotides. During an exonuclease digestion, rapid buildup in the concentration of nucleotides produces strong signal of nucleotide cluster ions
Autor:
Hoda Aboleneen, Huaiqin Wu
Publikováno v:
Analytical biochemistry. 287(1)
A method for sequencing ODNs with both termini blocked using mass spectrometry (MS) is reported. The ladder sequencing method is based on our investigation and understanding of critical factors affecting snake venom phosphodiesterase (SVP) digestion
Publikováno v:
Rapid communications in mass spectrometry : RCM. 14(1)
Fluorescein-labeled oligodeoxynucleotides (ODNs) from automated synthesis commonly produce multiple peaks in high performance liquid chromatography (HPLC) chromatograms. We found that these peaks are due to chemical modifications of the ODNs instead
Autor:
Qingling Zhang, Victor W. Armstrong, Christa Scholz, Michael Oellerich, Hoda Aboleneen, Maria Shipkova, Ekkehard Schuetz, Stephan Groothuisen
Publikováno v:
Clinical chemistry. 44(12)
A modified pentamer formation assay (PFA) for quantification of tacrolimus and active metabolites after extraction from whole blood is described. The lower limit of detection was 2 μg/L. Intraassay precision (CV) was 5.7–13.7%, and the interassay