Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Hocine Siar"'
Publikováno v:
Molecules, Vol 29, Iss 15, p 3674 (2024)
It has been reported that the modification of immobilized glyoxyl–ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of the immobilized protease against hemoglobin, while the protease still maintained a reaso
Externí odkaz:
https://doaj.org/article/f6c9cbc388df43a5ab1ea479ab1e6937
Autor:
Oumaima Cherni, Diego Carballares, El Hocine Siar, Pedro Abellanas-Perez, Diandra de Andrades, Javier Rocha-Martin, Sellema Bahri, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 14, Iss 2, p 115 (2024)
The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate support, and aminated agarose (monoaminoethyl-N-aminoethyl) have been utilized. Both immobi
Externí odkaz:
https://doaj.org/article/8e49bc283013464e9b45b2e26a38b9cc
Autor:
Sabrina Ait Braham, El-Hocine Siar, Sara Arana-Peña, Diego Carballares, Roberto Morellon-Sterling, Hossein Bavandi, Diandra de Andrades, Jakub F. Kornecki, Roberto Fernandez-Lafuente
Publikováno v:
Molecules, Vol 26, Iss 4, p 968 (2021)
This paper aims to investigate the effects of some salts (NaCl, (NH4)2SO4 and Na2SO4) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three proteases, two glycosidases, and one laccase, penicillin G acylase
Externí odkaz:
https://doaj.org/article/407a73dcf6fd46a8b4ecdf174f4a9e25
Autor:
Sabrina Ait Braham, Roberto Morellon-Sterling, Roberto Fernandez-Lafuente, Diego Carballares, Sara Arana-Peña, El-Hocine Siar
Publikováno v:
ACS Sustainable Chemistry & Engineering. 9:7508-7518
Autor:
El Hocine Siar, Diego Carballares, Sabrina Ait Braham, Jakub F. Kornecki, Roberto Fernandez-Lafuente, Diandra de Andrades, Roberto Morellon-Sterling, Sara Arana-Peña, Hossein Bavandi
Publikováno v:
Process Biochemistry. 102:108-121
In this paper, the effect of glycerol at different concentrations and pH values on the stabilities of 13 immobilized enzymes has been analyzed. Among the enzymes, we have selected 5 lipases (immobilized on octyl or amino-glutaraldehyde supports), 3 p
Autor:
Mohammed Nasreddine Zidoune, El-Hocine Siar, Roberto Fernandez-Lafuente, Roberto Morellon-Sterling
Publikováno v:
International Journal of Biological Macromolecules. 144:419-426
A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities
Autor:
El-Hocine Siar, Sara Arana-Peña, Oveimar Barbosa, Mohammed Nasreddine Zidoune, Roberto Fernandez-Lafuente
Publikováno v:
Catalysts, Vol 8, Iss 4, p 149 (2018)
Ficin extract has been immobilized on different 4% aminated-agarose beads. Using just ion exchange, immobilization yield was poor and expressed activity did not surpass 10% of the offered enzyme, with no significant effects on enzyme stability. The t
Externí odkaz:
https://doaj.org/article/2b08a47ab4904744982050c4cd09185f
Autor:
Attia Hamadi, Ferial Aziza Benyahia-Krid, Amani Harkati, Abdellah Zikiou, Mohamed Nasser Eddine Zidoune, El Hocine Siar, Ouarda Aissaoui-Zitoun, Faiza Adoui, Halima Boughellout
The internal bonds created in milk gelation with chicken pepsin were approached by the use of dissociating agents-sodium dodecyl sulfate (SDS), urea, ethylenediamine tetraacetic acid (EDTA) and compared to those with rennet gel. Gels viscoelasticity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7d74964c335ea25b64eec2f50fba7f3
https://doi.org/10.9734/bpi/cravs/v3/1956f
https://doi.org/10.9734/bpi/cravs/v3/1956f
Autor:
Justo Pedroche, Roberto Fernandez-Lafuente, El-Hocine Siar, Mª del Carmen Millán, Roberto Morellon-Sterling, Diandra de Andrades, Sabrina Ait Braham
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
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1 Tabla.-- 17 Figuras
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary amine group during the immobilization: ethyl am
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary amine group during the immobilization: ethyl am
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3963d67afba429dba5cd0181d451c0b5
http://hdl.handle.net/10261/233020
http://hdl.handle.net/10261/233020
Autor:
Sabrina Ait, Braham, Roberto, Morellon-Sterling, Diandra, de Andrades, Rafael C, Rodrigues, El-Hocine, Siar, Ali, Aksas, Justo, Pedroche, Maria Del Carmen, Millán, Roberto, Fernandez-Lafuente
Publikováno v:
Applied biochemistry and biotechnology. 193(9)
Tris is an extensively used buffer that presents a primary amine group on its structure. In the present work trypsin, chymotrypsin and penicillin G acylase (PGA) were immobilized/stabilized on glyoxyl agarose in presence of different concentrations o