Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Ho Joung Lee"'
Publikováno v:
Frontiers in Genome Editing, Vol 6 (2024)
The paired nickases approach, which utilizes clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) nickase and dual guide RNA, has the advantage of reducing off-target effects by being able to double the
Externí odkaz:
https://doaj.org/article/9b205c72d68646b5a1d00f0367f30578
Publikováno v:
ACS synthetic biology. 11(11)
Enterobacterial phage λ is a temperate phage that infects
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::af6081cdcfa18b908ca06b79a3d00cbb
https://pubmed.ncbi.nlm.nih.gov/36326101
https://pubmed.ncbi.nlm.nih.gov/36326101
Publikováno v:
Journal of Microbiology. 61:13-36
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::17b8135e5a8ad2903ccebc6601840bba
https://doi.org/10.1007/s12275-022-00005-5
https://doi.org/10.1007/s12275-022-00005-5
Publikováno v:
ACS Synthetic Biology. 11:3829-3835
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::653d9ddc7b9ddb1500c4bf91019c3812
https://doi.org/10.1021/acssynbio.2c00409
https://doi.org/10.1021/acssynbio.2c00409
Publikováno v:
The CRISPR journal.
The CRISPR-Cas system has been used as a convenient tool for genome editing because the nuclease that cuts the target DNA and the guide RNA that recognizes the target are separated into modules. Cas12f1, which has a smaller size than that of other Ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0eaba96e13f3cf2a6a5b50e962cf218
https://pubmed.ncbi.nlm.nih.gov/36576897
https://pubmed.ncbi.nlm.nih.gov/36576897
Autor:
Ho Joung Lee, Sang Jun Lee
Publikováno v:
Journal of Microbiology and Biotechnology. 31:903-911
Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68b8f60c6e6821fe15e67069f0a63bd6
https://doi.org/10.4014/jmb.2106.06056
https://doi.org/10.4014/jmb.2106.06056
Publikováno v:
ACS synthetic biology. 11(6)
Mismatch tolerance, a cause of the off-target effect, impedes accurate genome editing with the CRISPR/Cas system. Herein, we observed that oligonucleotide-directed single-base substitutions could be rarely introduced in the microbial genome using CRI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e5f865aa48fb0db7927a57a3d604f25
https://pubmed.ncbi.nlm.nih.gov/35584409
https://pubmed.ncbi.nlm.nih.gov/35584409
Determination of the Length of Target Recognition Sequence in sgRNA Required for CRISPR Interference
Publikováno v:
Microbiology and Biotechnology Letters.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3738c2d34263c34e774639ee8eac8c83
https://doi.org/10.48022/mbl.2111.11003
https://doi.org/10.48022/mbl.2111.11003
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 6457, p 6457 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 12
International Journal of Molecular Sciences
Volume 22
Issue 12
The CRISPR/Cas9 system has recently emerged as a useful gene-specific editing tool. However, this approach occasionally results in the digestion of both the DNA target and similar DNA sequences due to mismatch tolerance, which remains a significant d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c5c6a111f1ed6615233f6a445ff5aac
https://www.mdpi.com/1422-0067/22/12/6457
https://www.mdpi.com/1422-0067/22/12/6457
Publikováno v:
Genome Res
Genome editing has been revolutionized by the CRISPR-Cas9 system. CRISPR-Cas9 is composed of single-molecular guide RNA (sgRNA) and a proteinaceous Cas9 nuclease, which recognizes a specific target sequence and a protospacer adjacent motif (PAM) sequ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af83f5e5c8cc2379d1bdf8cd559e7f0c
https://pubmed.ncbi.nlm.nih.gov/32327447
https://pubmed.ncbi.nlm.nih.gov/32327447