The interplay between peptides and RNA is critical for protoribosome compartmentalization and stability.

Autor: Codispoti S; Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università di Milano, Segrate 20054, Italy., Yamaguchi T; Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.; Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 12800, Czech Republic., Makarov M; Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 12800, Czech Republic., Giacobelli VG; Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 12800, Czech Republic., Mašek M; Department of Physical Chemistry, University of Chemistry and Technology, Technicka 5, 16628 Prague, Czech Republic., Kolář MH; Department of Physical Chemistry, University of Chemistry and Technology, Technicka 5, 16628 Prague, Czech Republic., Sanchez Rocha AC; Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 12800, Czech Republic., Fujishima K; Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.; Graduate School of Media and Governance, Keio University, 5322 Endo, Fujisawa 252-0882, Japan., Zanchetta G; Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università di Milano, Segrate 20054, Italy., Hlouchová K; Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 12800, Czech Republic.; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo n. 2, Prague 16610, Czech Republic.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2024 Nov 11; Vol. 52 (20), pp. 12689-12700.

Experimental characterization of de novo proteins and their unevolved random-sequence counterparts.

Autor: Heames B; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Buchel F; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic.; Department of Biochemistry, Charles University, Prague, Czech Republic., Aubel M; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Tretyachenko V; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic., Loginov D; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Novák P; Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic., Lange A; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany., Bornberg-Bauer E; Institute for Evolution and Biodiversity, University of Münster, Münster, Germany. ebb@wwu.de.; Department of Protein Evolution, MPI for Developmental Biology, Tübingen, Germany. ebb@wwu.de., Hlouchová K; Department of Cell Biology, Charles University, BIOCEV, Prague, Czech Republic. klara.hlouchova@natur.cuni.cz.; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic. klara.hlouchova@natur.cuni.cz.

Publikováno v: Nature ecology & evolution [Nat Ecol Evol] 2023 Apr; Vol. 7 (4), pp. 570-580. Date of Electronic Publication: 2023 Apr 06.

Expression of glutamate carboxypeptidase II in human brain

Autor: Šácha, P., Zámečník, J., Bařinka, C., Hlouchová, K., Vícha, A., Mlčochová, P., Hilgert, I., Eckschlager, T., Konvalinka, J.

Publikováno v: In Neuroscience 2007 144(4):1361-1372

Modern and prebiotic amino acids support distinct structural profiles in proteins.

Autor: Tretyachenko V; Department of Cell Biology, Faculty of Science, Charles University, Prague 12843, Czech Republic.; Department of Biochemistry, Faculty of Science, Charles University, Prague 12843, Czech Republic., Vymětal J; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague 16610, Czech Republic., Neuwirthová T; Department of Cell Biology, Faculty of Science, Charles University, Prague 12843, Czech Republic., Vondrášek J; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague 16610, Czech Republic., Fujishima K; Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo 1528550, Japan.; Graduate School of Media and Governance, Keio University, Fujisawa 2520882 Japan., Hlouchová K; Department of Cell Biology, Faculty of Science, Charles University, Prague 12843, Czech Republic.; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague 16610, Czech Republic.

Publikováno v: Open biology [Open Biol] 2022 Jun; Vol. 12 (6), pp. 220040. Date of Electronic Publication: 2022 Jun 22.

Enzyme catalysis prior to aromatic residues: Reverse engineering of a dephospho-CoA kinase.

Autor: Makarov M; Department of Cell Biology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.; Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic., Meng J; Department of Biochemistry and Molecular Biology, Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, USA., Tretyachenko V; Department of Cell Biology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.; Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic., Srb P; Institute of Organic Chemistry and Biochemistry, IOCB Research Centre & Gilead Sciences, Academy of Sciences of the Czech Republic, Prague, Czech Republic., Březinová A; Proteomics Core Facility, BIOCEV, Faculty of Science, Charles University, Prague, Czech Republic., Giacobelli VG; Department of Cell Biology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic., Bednárová L; Institute of Organic Chemistry and Biochemistry, IOCB Research Centre & Gilead Sciences, Academy of Sciences of the Czech Republic, Prague, Czech Republic., Vondrášek J; Institute of Organic Chemistry and Biochemistry, IOCB Research Centre & Gilead Sciences, Academy of Sciences of the Czech Republic, Prague, Czech Republic., Dunker AK; Department of Biochemistry and Molecular Biology, Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, USA., Hlouchová K; Department of Cell Biology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic.; Institute of Organic Chemistry and Biochemistry, IOCB Research Centre & Gilead Sciences, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

Publikováno v: Protein science : a publication of the Protein Society [Protein Sci] 2021 May; Vol. 30 (5), pp. 1022-1034. Date of Electronic Publication: 2021 Mar 26.