Zobrazeno 1 - 10
of 391
pro vyhledávání: '"Hitoshi SHIKU"'
Autor:
Kosuke Ino, An Konno, Yoshinobu Utagawa, Taiyo Kanno, Kazuyuki Iwase, Hiroya Abe, Hitoshi Shiku
Publikováno v:
Micromachines, Vol 15, Iss 8, p 1054 (2024)
Two-layer microfluidic devices with porous membranes have been widely used in bioapplications such as microphysiological systems (MPS). Porous electrodes, instead of membranes, have recently been incorporated into devices for electrochemical cell ana
Externí odkaz:
https://doaj.org/article/498032c129804e4d88124999e9d958c3
Autor:
Ayane ENDO, Koki KUBOTA, Takahiro ITO-SASAKI, Mayo KOMATSU, Tomoki IWAMA, Hitoshi SHIKU, Kumi Y. INOUE
Publikováno v:
Electrochemistry, Vol 92, Iss 2, Pp 022013-022013 (2024)
Carbon paste electrodes (CPEs) are widely used because of their malleability and ease of modification of functional molecules. This study introduces the application of Au paste electrodes (AuPEs) as a method for amplifying electrochemiluminescence (E
Externí odkaz:
https://doaj.org/article/66e55c0b43684cd4a1e41dcc9966b4df
Publikováno v:
Electrochemistry, Vol 92, Iss 2, Pp 022009-022009 (2024)
Cell adhesion to culture substrates plays a crucial role in cellular activities, such as proliferation, differentiation, and apoptosis. Recently, electrochemiluminescence (ECL) imaging has been utilized for analyzing cell adhesion. In addition to ECL
Externí odkaz:
https://doaj.org/article/831d8fd95cd7419dbefbe4c02ae62d65
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Heterogeneous nature is a pivotal aspect of cancer, rendering treatment problematic and frequently resulting in recurrence. Therefore, advanced techniques for identifying subpopulations of a tumour in an intact state are essential to develop novel sc
Externí odkaz:
https://doaj.org/article/d286aac2c91644aea7ce868ab33afa14
Publikováno v:
Biosensors and Bioelectronics: X, Vol 13, Iss , Pp 100312- (2023)
Here, we report a droplet-free digital immunoassay for goat immunoglobulin G (IgG) based on electrochemiluminescence (ECL) for simplified digital immunoassay. Digital enzyme-linked immunosorbent assay (ELISA) enabled ultrasensitive detection using di
Externí odkaz:
https://doaj.org/article/52b3938f23e4496486a3585544ed1a0c
Publikováno v:
ACS Omega, Vol 6, Iss 51, Pp 35476-35483 (2021)
Externí odkaz:
https://doaj.org/article/53b0f040318845fd89d1ef02c289b0bb
Publikováno v:
Electrochemistry, Vol 89, Iss 5, Pp 409-414 (2021)
In this work, we report a simple electrochemical sensor that detects proteinuria upon immersion into a sample. We immobilized peptidases in conjunction with glutamate oxidase (GluOx) on a Prussian blue-modified glassy carbon (PB-GC) electrode. In the
Externí odkaz:
https://doaj.org/article/70ca8ec8e1ac423f992c8708185493e5
Publikováno v:
Electrochemical Science Advances, Vol 2, Iss 5, Pp n/a-n/a (2022)
Abstract Vascular form vascular organs and the walls of blood vessels play a crucial role in transporting nutritive compounds and drugs. Vascular cells also release factors required to maintain the homeostasis of the organs. Since cancer cells travel
Externí odkaz:
https://doaj.org/article/1c2917dcd4cf4a3687fa98eb1d921626
Autor:
Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku
Publikováno v:
Micromachines, Vol 14, Iss 2, p 327 (2023)
The Limulus amebocyte lysate (LAL) reaction-based assay, the most commonly used endotoxin detection method, requires a skilled technician. In this study, to develop an easy-to-use and highly sensitive endotoxin sensor, we created an electrochemical e
Externí odkaz:
https://doaj.org/article/9a8074a3276d4ee398d80ca338deafb2
Autor:
Yoshinobu Utagawa, Kosuke Ino, Tatsuki Kumagai, Kaoru Hiramoto, Masahiro Takinoue, Yuji Nashimoto, Hitoshi Shiku
Publikováno v:
Micromachines, Vol 13, Iss 3, p 420 (2022)
Three-dimensional organs and tissues can be constructed using hydrogels as support matrices for cells. For the assembly of these gels, chemical and physical reactions that induce gluing should be induced locally in target areas without causing cell d
Externí odkaz:
https://doaj.org/article/be0eb860031d405686a430fc3e3e5f74