Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Hitoshi Furusho"'
Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX
Autor:
Koji Wakui, Toru Yoshitomi, Akane Yamaguchi, Maho Tsuchida, Shingo Saito, Masami Shibukawa, Hitoshi Furusho, Keitaro Yoshimoto
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 16, Iss , Pp 348-359 (2019)
We present a rapidly neutralizable and highly anticoagulant thrombin-binding aptamer with a short toehold sequence, originally discovered by systematic evolution of ligands by exponential enrichment (SELEX) with microbead-assisted capillary electroph
Externí odkaz:
https://doaj.org/article/8663669d85c242abadea8927601286e9
Autor:
Takumi Oguro, Hitoshi Furusho, Keiko Kimura, Fumiya Wayama, Keitaro Yoshimoto, Misako Hayashi, Toru Yoshitomi
Publikováno v:
Molecular Therapy. Nucleic Acids
Vascular endothelial growth factors (VEGFs) are hypoxia-inducible secreted proteins to promote angiogenesis, in which VEGF-A is an important molecule that binds and activates VEGF receptor-1 (VEGFR-1) and VEGFR-2. In this study, two DNA aptamers, Apt
Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX
Autor:
Maho Tsuchida, Keitaro Yoshimoto, Shingo Saito, Koji Wakui, Hitoshi Furusho, Akane Yamaguchi, Toru Yoshitomi, Masami Shibukawa
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 16, Iss, Pp 348-359 (2019)
Molecular Therapy. Nucleic Acids
Molecular Therapy. Nucleic Acids
We present a rapidly neutralizable and highly anticoagulant thrombin-binding aptamer with a short toehold sequence, originally discovered by systematic evolution of ligands by exponential enrichment (SELEX) with microbead-assisted capillary electroph
Publikováno v:
Analytical Sciences. 35:585-588
Nucleobase-modified aptamers are attractive candidates for diagnostic and therapeutic agents due to the high affinity, stability and functionality. However, since even conventional SELEX requires many selection rounds, acquisition of modified aptamer
Publikováno v:
Analytical Sciences. 35:113-116
Here, we demonstrated a strategy for developing signaling aptamers, based on screening of signaling aptamers from multiple aptamer candidates obtained by SELEX with next generation sequencing. Among aptamer candidates labelled by 6-carboxyfluorescein
Autor:
Huabing Yin, Tianxin Yang, Hitoshi Furusho, Jianan Yin, Yuanshuai Zhu, Andrew Glidle, Yuchen Fu, Yingkai Lyu
Publikováno v:
Integrated Sensors for Biological and Neural Sensing.
Microflow cytometry has become an attractive tool for counting and analysis of complex cell population and finds many applications in life science research. However, its implementation in screening small sized targets, such as microorganisms, is limi
Publikováno v:
Microfluidics and Nanofluidics. 25
Optical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ t
Autor:
Hitoshi Furusho, Andrew Glidle, Yuchen Fu, Xiaofei Yuan, Tianxin Yang, Huabing Yin, Yingkai Lyu
Publikováno v:
Lab on a chip. 20(22)
Raman activated cell sorting has emerged as a label-free technology that can link phenotypic function with genotypic properties of cells. However, its broad implementation is limited by challenges associated with throughput and the complexity of biol
Autor:
Keitaro Yoshimoto, Ryo Maruyama, Hiroharu Yui, Hitoshi Furusho, Toru Yoshitomi, Kurumi Makino
Publikováno v:
The Analyst. 143:4022-4026
Since much attention has been paid to in vivo biological functions of G-quadruplexes, structural analyses of G-quadruplexes are essential for understanding their functional mechanisms. Here, we established a simple optical-spectroscopy-based method f
Publikováno v:
Biomaterials. 134
Early diagnosis of metastatic cancers could greatly limit the number of cancer-associated deaths. Aberrant surface expression of sialic acid (hypersialylation) on tumors correlating with metastatic incidence and its involvement in tumorigenesis and p