Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Hisashi Yasui"'
Publikováno v:
Journal of Biochemistry. 123:827-831
We examined temperature dependency of thermodynamic parameters in the interactions between hen egg-white lysozyme (HEL) and anti-HEL antibody, D1.3, and two mutant antibodies. The DeltaH degrees values appeared to decrease biphasically in the tempera
Publikováno v:
FEBS Letters. 353:143-146
The thermal stability of Fv fragments was examined by circular dichroism (CD) spectrometry and high-performance liquid chromatography. We analyzed three Fv fragments: that of a monoclonal antibody D1.3 and two derivatives of it. After separation of w
Autor:
Yoshikazu Kurosawa, Hisashi Yasui
Publikováno v:
Gene. 129:135-139
We measured the frequencies of recombination in a yeast host between two homologous segments of DNA that had been inserted with the same polarity in a yeast artificial chromosome (YAC) vector. Three kinds of YAC clones were constructed in which the g
Publikováno v:
Journal of molecular biology. 248(4)
Effects of mutations on antigen-antibody interactions were energetically examined using several kinds of Fv fragments, derivatives of a monoclonal antibody, D1.3, that is specific for hen egg-white lysozyme. According to the transition-state theory,
Autor:
Takashi Tsuruo, Yoshikazu Kurosawa, Hisashi Yasui, Tetsuo Mashima, Yoshihisa Kuwana, Mikihiko Naito, Hiroyuki Seimiya
Publikováno v:
Journal of biochemistry. 113(6)
The T cell receptor alpha and beta chains are covalently linked via a disulfide bond in their extracellular constant regions. To use these domains as specific hetero-cross-linkers of two different polypeptides, we created genetic constructs encoding
Autor:
Shoichi Shimomura, Tomoko Sumi, Miwako Takahashi, Toyomi Shibata, Haruko Kato, Noriji Suzuki, Hisao Fukushima, Yasuo Yamada, Akemi Ito, Eizi Nakayama, Toshihiro Osuka, Hisashi Yasui, Hisako Tanaka
Publikováno v:
JOURNAL OF THE JAPANESE ASSOCIATION OF RURAL MEDICINE. 36:923-927
愛知県海部郡一帯では水田作業中の農民に約40年前から鳥類住血吸虫セルカリアによる皮膚炎が発症していることが知られているが、ヒラマキモドキからGigantobilharzia属のセルカリアおよ
Autor:
Ikuya Tsuge, Jun-ichi Okada, Yoshikazu Ichihara, Hiroshi Matsuoka, Hisashi Yasui, Shinpei Torii, Yoshikazu Kurosawa
Publikováno v:
Immunogenetics. 27:330-337
In an attempt to characterize the genes that cause immunodeficiencies such as X-linked agammaglobulinemia (XLA) and severe combined immunodeficiency (SCID) we established precursor B-cell lines by transforming the patients' bone marrow cells with Eps
Autor:
Masumi Abe, Makoto Hibiya, Hiroshi Matsuoka, Kazuhiko Okamura, Morihiro Naito, Hiroshi Handa, Kenji Imai, Hisashi Yasui, Yasushi Akahori, Yoshikazu Kurosawa, Koh-zoh Kameyama
Publikováno v:
Nucleic Acids Research. 16:9497-9511
Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the mu-delta intron, two repeating units were contained. One 442-base-long fragment located JH-mu intron (defined
Publikováno v:
European Journal of Immunology. 19:1399-1403
Class switch of immunoglobulin from mu to gamma occurs by recombination between two repetitive switch sequences: S mu and S gamma. However, there are no such sequences in the mu-delta introns of human and mouse genomes. Although the frequency of IgD-
Publikováno v:
European journal of immunology. 18(4)
The variable region of immunoglobulin (Ig) heavy chain is encoded by three separate genes: variable (VH), diversity (DH) and joining (JH) genes on the germ-line genome. In mice, most complementarity determining region (CDR) 111 of the heavy chains of