Zobrazeno 1 - 10
of 86
pro vyhledávání: '"Hisao Moriya"'
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 12, Iss 6 (2022)
AbstractThe enhanced green fluorescent protein (EGFP) is considered to be a harmless protein because the critical expression level that causes growth defects is higher than that of other proteins. Here, we found that overexpression of EGFP, but not a
Externí odkaz:
https://doaj.org/article/f8d33a7d1b664e22812d3b33664acfcd
Autor:
Hisao Moriya
Publikováno v:
AIMS Biophysics, Vol 7, Iss 2, Pp 121-132 (2020)
Nucleotide and amino acid sequences at the N-terminus affect the expression level and cytotoxicity of proteins; however, their effects are not fully understood yet. Here, N-terminal 30 nucleotide/10 amino acid (N10) sequences that affect the expressi
Externí odkaz:
https://doaj.org/article/5207dd73addb47158f51b1f83c33a7e1
Autor:
Reiko Kintaka, Koji Makanae, Shotaro Namba, Hisaaki Kato, Keiji Kito, Shinsuke Ohnuki, Yoshikazu Ohya, Brenda J Andrews, Charles Boone, Hisao Moriya
Publikováno v:
eLife, Vol 9 (2020)
Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein bu
Externí odkaz:
https://doaj.org/article/e6787edb0fe14313bbb3ee62e8ff839b
Publikováno v:
PLoS Genetics, Vol 16, Iss 10, p e1009091 (2020)
Proper control of gene expression levels upon various perturbations is a fundamental aspect of cellular robustness. Protein-level dosage compensation is one mechanism buffering perturbations to stoichiometry of multiprotein complexes through accelera
Externí odkaz:
https://doaj.org/article/4a068823a32e49b582b1e0560931555b
Publikováno v:
eLife, Vol 7 (2018)
The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically meas
Externí odkaz:
https://doaj.org/article/e0a64d5f20db440ca7ad074a50a78832
Publikováno v:
PLoS Genetics, Vol 13, Iss 1, p e1006554 (2017)
Understanding buffering mechanisms for various perturbations is essential for understanding robustness in cellular systems. Protein-level dosage compensation, which arises when changes in gene copy number do not translate linearly into protein level,
Externí odkaz:
https://doaj.org/article/3a8f2cf170504a45ab038a58c5a85936
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0120678 (2015)
In a previous study, we found an unknown element that caused growth inhibition after its copy number increased in the 3' region of DIE2 in Saccharomyces cerevisiae. In this study, we further identified this element and observed that overexpression of
Externí odkaz:
https://doaj.org/article/33302f2d4ebb49939be9145b860e29fa
Publikováno v:
PLoS ONE, Vol 8, Iss 9, p e73319 (2013)
We previously determined the copy number limits of overexpression for cell division cycle (cdc) regulatory genes in the fission yeast Schizosaccharomyces pombe using the "genetic tug-of-war" (gTOW) method. In this study, we measured the levels of tan
Externí odkaz:
https://doaj.org/article/ea197515343e4fd5a539f7f04c0cecc7
Publikováno v:
PLoS Genetics, Vol 6, Iss 4, p e1000919 (2010)
Cells can maintain their functions despite fluctuations in intracellular parameters, such as protein activities and gene expression levels. This commonly observed biological property of cells is called robustness. On the other hand, these parameters
Externí odkaz:
https://doaj.org/article/ac50fe8147a74b37abcf31c5f7c92d21
Publikováno v:
PLoS ONE, Vol 5, Iss 3, p e9652 (2010)
BackgroundConstruction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligas
Externí odkaz:
https://doaj.org/article/e34cf0b707bf4f6a96ba9e6be0dd36da