Zobrazeno 1 - 10
of 91
pro vyhledávání: '"Hiroyuki Kajiura"'
Autor:
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Publikováno v:
Frontiers in Plant Science, Vol 14 (2023)
N-glycan engineering has dramatically evolved for the development and quality control of recombinant antibodies. Fc region of IgG contains two N-glycans whose galactose terminals on Fc-glycan have been shown to increase the stability of CH2 domain an
Externí odkaz:
https://doaj.org/article/41a31557575945e393caa5e5815afe6b
Autor:
Hiroyuki Kajiura, Ken-ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)
Abstract The silkworm, Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production in B. mori: bac
Externí odkaz:
https://doaj.org/article/9b9a16dad20a4bc99efd5c515a91f0db
Autor:
Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract N-Glycosylation is one of the most important post-translational protein modifications in eukaryotic cells. Although more than 200 N-glycogenes contributing to N-glycan biosynthesis have been identified and characterized, the information on i
Externí odkaz:
https://doaj.org/article/66dc3cfbc89c474a86596a4dccf9a055
Autor:
Hiroyuki Kajiura, Takuya Yoshizawa, Yuji Tokumoto, Nobuaki Suzuki, Shinya Takeno, Kanokwan Jumtee Takeno, Takuya Yamashita, Shun-ichi Tanaka, Yoshinobu Kaneko, Kazuhito Fujiyama, Hiroyoshi Matsumura, Yoshihisa Nakazawa
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Kajiura and Yoshizawa et al. identify three new prenyltransferases in the tree Eucommia ulmoides that synthesize exceptionally high molecular weight trans-1,4-polyisoprene (TPI). Through crystal structure and mutational analyses, they identify key re
Externí odkaz:
https://doaj.org/article/0d8d4e20f5c54474bdd6cf4c60bfba98
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium. However, the use of this platform i
Externí odkaz:
https://doaj.org/article/003a591b60cb433c84cbb38892ee6f9f
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase). Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease. Importantly, the terminal ma
Externí odkaz:
https://doaj.org/article/e7ab9fd5b00e428fa6a5ba8d630eb677
Autor:
Bussarin Wachananawat, Takeshi Kuroha, Yuto Takenaka, Hiroyuki Kajiura, Satoshi Naramoto, Ryusuke Yokoyama, Kimitsune Ishizaki, Kazuhiko Nishitani, Takeshi Ishimizu
Publikováno v:
Frontiers in Plant Science, Vol 11 (2020)
Rhamnogalacturonan I (RG-I) comprises approximately one quarter of the pectin molecules in land plants, and the backbone of RG-I consists of a repeating sequence of [2)-α-L-Rha(1-4)-α-D-GalUA(1-] disaccharide. Four Arabidopsis thaliana genes encodi
Externí odkaz:
https://doaj.org/article/bbba1cbcc6ec4f65adc4bc5c3e9342da
Autor:
Sugata Roychowdhury, Young J. Oh, Hiroyuki Kajiura, Krystal T. Hamorsky, Kazuhito Fujiyama, Nobuyuki Matoba
Publikováno v:
Frontiers in Plant Science, Vol 9 (2018)
Nicotiana benthamiana transient overexpression systems offer unique advantages for rapid and scalable biopharmaceuticals production, including high scalability and eukaryotic post-translational modifications such as N-glycosylation. High-mannose-type
Externí odkaz:
https://doaj.org/article/b0ac2cb3e51947849ce83560066ec604
Autor:
Krystal Teasley Hamorsky, J Calvin Kouokam, Lauren J Bennett, Keegan J Baldauf, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2046 (2013)
INTRODUCTION: Cholera toxin B subunit (CTB) is a component of an internationally licensed oral cholera vaccine. The protein induces neutralizing antibodies against the holotoxin, the virulence factor responsible for severe diarrhea. A field clinical
Externí odkaz:
https://doaj.org/article/a1d061edec6c445484c017d2acc5a6bc
Autor:
John Benson Dy Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki
Publikováno v:
Cytotechnology. 75:219-229
Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of the medium is an effective approach to improving the production and quality of recombinant proteins.