Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Hirotaka Miyachi"'
Publikováno v:
Biosensors and Bioelectronics. 25:1395-1401
Episodes of shellfish contamination with okadaic acid (OA) are a human health threat that is causing increasing concern. As a way to overcome the shortcomings involved in the reference methods of analysis set by legislations, alternative procedures a
Autor:
Hyen-Wook Kang, Tsubasa Shibuya, Tomoko Okada, Hiroshi Muramatsu, Jong-Min Kim, Hirotaka Miyachi, Yuji Yamamoto, Isao Karube
Publikováno v:
Biochemical Engineering Journal. 41:17-23
We have developed a new method for evaluating the affinity interactions between two different proteins by applying an alternating current (AC) voltage to a micro-flow channel. An AC voltage was applied to the protein-modified microspheres in the micr
Publikováno v:
Biosensors and Bioelectronics. 22:1480-1486
This study proved a possibility of a peptide probe for evaluating affinity properties of proteins. We have designed and synthesized three different peptide probes, H-Ala3-(Gly-Pro5)3-Gly-OH (peptide A), H-Ala3-(Gly-Pro5)-Gly-OH (peptide B) and H-Ala3
Publikováno v:
NanoBiotechnology. 2:117-121
The DNA-binding protein (DBP) has a wide range of roles such as those in DNA repair, recombination, and gene expression. Recently, a microarray-based method has been developed for the high-throughput analysis of DNA-protein interactions. However, to
Publikováno v:
The Journal of Biochemistry. 138:473-478
The study of DNA-protein interactions is of great importance to understand basic cellular processes such as transcription, replication and recombination. In this research, we developed a novel detection system for DNA-binding proteins (DBPs) involvin
Publikováno v:
Biosensors and Bioelectronics. 20:1091-1097
A method of preparing a thin polymer layer able to recognize double-stranded DNA (dsDNA) was developed by using 2-vinyl-4,6-diamino-1,3,5-triazine (VDAT) as a functional monomer for creating a DNA-imprinted polymer. The formation of hydrogen bonds be
Autor:
Isao Karube, Kazunori Ikebukuro, Hirotaka Miyachi, Sadayori Hoshina, Midori Kono, Kazuyoshi Yano
Publikováno v:
Analytica Chimica Acta. 407:1-10
Chimeric oligonucleotides consisting of 21 bases of ribonucleic acid (RNA) with six bases of deoxyribonucleic acid (DNA) at the 3′-hydroxyl terminus (chimeric RNA–DNA primer) and the recombinant thermostable DNA polymerase derived from Thermus th
Autor:
Hirotaka Miyachi, Kazunori Ikebukuro, Hideo Ohkawa, Satoshi Sasaki, Isao Karube, Eriko Kai, Hitoshi Muguruma
Publikováno v:
Analytica Chimica Acta. 363:229-233
Direct determination of etofenprox was performed using surface plasmon resonance. A thin gold film sputtered on to a glass surface was coated with plasma polymerized ethylene diamine (1,2-diaminoethane). Polyclonal anti-etofenprox antibody was covale
Synthesis and structure-activity relationship of tricyclic carboxylic acids as novel anti-histamines
Autor:
Masako Onishi, Hirotaka Miyachi, Hirotaka Kurebayashi, Masanori Tobe, Yoshiaki Isobe, Katsumi Kubota
Publikováno v:
Bioorganicmedicinal chemistry. 19(9)
A series of tricyclic carboxylic acids having 6-amino-pyrimidine-2,4(1H,3H)-dione with piperazino or homopiperazino moiety linked by propylene, were synthesized and evaluated for their affinity toward human histamine H1 receptor and Caco-2 cell perme
Autor:
Hirotaka Kurebayashi, Masanori Tobe, Katsumi Kubota, Masako Onishi, Hirotaka Miyachi, Yoshiaki Isobe
Publikováno v:
Bioorganicmedicinal chemistry letters. 19(10)
A series of phenothiazine carboxylic acid derivatives, having 6-amino-pyrimidine-2,4(1H,3H)-dione moiety via a appropriate linker, were synthesized and evaluated for their affinity toward human histamine H1 receptor and Caco-2 cell permeability. Sele