Zobrazeno 1 - 10
of 111
pro vyhledávání: '"Hiroshi Nishimasu"'
Autor:
Toshihiro Nakane, Ryoya Nakagawa, Soh Ishiguro, Sae Okazaki, Hideto Mori, Yutaro Shuto, Keitaro Yamashita, Nozomu Yachie, Hiroshi Nishimasu, Osamu Nureki
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-10 (2024)
Abstract The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets complementary to an RNA guide, and is widely used as a powerful genome-editing tool. Here, we report the crystal structure of Brevibacillus laterosporus Cas9 (BlCas9, a
Externí odkaz:
https://doaj.org/article/783bfe326ea64e06babe63f8818ac743
Autor:
Sundaram Acharya, Asgar Hussain Ansari, Prosad Kumar Das, Seiichi Hirano, Meghali Aich, Riya Rauthan, Sudipta Mahato, Savitri Maddileti, Sajal Sarkar, Manoj Kumar, Rhythm Phutela, Sneha Gulati, Abdul Rahman, Arushi Goel, C. Afzal, Deepanjan Paul, Trupti Agrawal, Vinay Kumar Pulimamidi, Subhadra Jalali, Hiroshi Nishimasu, Indumathi Mariappan, Osamu Nureki, Souvik Maiti, Debojyoti Chakraborty
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-23 (2024)
Abstract The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins. The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for mismatched substrates, but its low cellular targeting e
Externí odkaz:
https://doaj.org/article/967b3513bca24917a4d3b9306cf6ba0f
PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells
Autor:
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Publikováno v:
Communications Medicine, Vol 3, Iss 1, Pp 1-12 (2023)
Abstract Background Base editing via CRISPR-Cas9 has garnered attention as a method for correcting disease-specific mutations without causing double-strand breaks, thereby avoiding large deletions and translocations in the host chromosome. However, i
Externí odkaz:
https://doaj.org/article/8d87658f0b3149538b80ca60fb8ca67c
Autor:
Kazuki Kato, Sae Okazaki, Soumya Kannan, Han Altae-Tran, F. Esra Demircioglu, Yukari Isayama, Junichiro Ishikawa, Masahiro Fukuda, Rhiannon K. Macrae, Tomohiro Nishizawa, Kira S. Makarova, Eugene V. Koonin, Feng Zhang, Hiroshi Nishimasu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
The cryo-EM structure of the IscB–ωRNA–target DNA complex provide insights into the ωRNA-guided DNA cleavage mechanism by IscB and the evolution of the type II CRISPR-Cas9 effector complexes
Externí odkaz:
https://doaj.org/article/f0ba686eba53416aba3c9403c2e20cc5
Autor:
Setsuko Mise-Omata, Makoto Ando, Tanakorn Srirat, Kensuke Nakagawara, Taeko Hayakawa, Mana Iizuka-Koga, Hiroshi Nishimasu, Osamu Nureki, Minako Ito, Akihiko Yoshimura
Publikováno v:
Cell Reports, Vol 42, Iss 8, Pp 112940- (2023)
Summary: Interleukin (IL)-6 is abundantly expressed in the tumor microenvironment and is associated with poor patient outcomes. Here, we demonstrate that the deletion of the suppressor of cytokine signaling 3 (SOCS3) in T cells potentiates anti-tumor
Externí odkaz:
https://doaj.org/article/3f935b2e37374fd2b1dbfe62d4e7a10b
Autor:
Hajime Shinoda, Tatsuya Iida, Asami Makino, Mami Yoshimura, Junichiro Ishikawa, Jun Ando, Kazue Murai, Katsumi Sugiyama, Yukiko Muramoto, Masahiro Nakano, Kotaro Kiga, Longzhu Cui, Osamu Nureki, Hiroaki Takeuchi, Takeshi Noda, Hiroshi Nishimasu, Rikiya Watanabe
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-8 (2022)
An automated amplification-free digital RNA detection platform using CRISPR-Cas13a and magnetic bead technology implemented in a microchamber device demonstrates sensitive SARS-CoV-2 genomic RNA detection and variant discrimination.
Externí odkaz:
https://doaj.org/article/923ad554159546cdac4a486c9fc8e071
Autor:
Ryoya Nakagawa, Soh Ishiguro, Sae Okazaki, Hideto Mori, Mamoru Tanaka, Hiroyuki Aburatani, Nozomu Yachie, Hiroshi Nishimasu, Osamu Nureki
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-8 (2022)
SpCas9 is a versatile genome-editing tool, but it is large and cannot be packaged efficiently in a AAV vector, limiting its application. The reported engineered Campylobacter jejuni Cas9 variant exhibits enhanced cleavage activity and a broader targe
Externí odkaz:
https://doaj.org/article/af853ca776f84a4fb19d26f881471e55
Autor:
Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong-Seok Oh, Dong Young Kim, Jae-Sung Woo, Hiroshi Nishimasu, Sung-Rae Cho, Sungroh Yoon, Hyongbum Henry Kim
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
While prime editing is a promising technology, PE2 systems often have low efficiency. Here the authors fuse a Rad51 DNA-binding domain to create hyPE2 with improved editing efficiency.
Externí odkaz:
https://doaj.org/article/585d27be31a74bf5926e523fa4034081
Autor:
Seiya Oura, Taichi Noda, Naoko Morimura, Seiji Hitoshi, Hiroshi Nishimasu, Yoshitaka Nagai, Osamu Nureki, Masahito Ikawa
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Seiya Oura and Taichi Noda et al. overcome the challenge of gene editing in CAG repeats, such as those causing Huntington’s Disease, using their recently developed SpCas9-NG variant. They demonstrate that SpCas9-NG can precisely edit and contract t
Externí odkaz:
https://doaj.org/article/6b8b24cc62624b4fbbeec478f8cf925c
Autor:
Hajime Shinoda, Yuya Taguchi, Ryoya Nakagawa, Asami Makino, Sae Okazaki, Masahiro Nakano, Yukiko Muramoto, Chiharu Takahashi, Ikuko Takahashi, Jun Ando, Takeshi Noda, Osamu Nureki, Hiroshi Nishimasu, Rikiya Watanabe
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-7 (2021)
The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a proof-of-concept, they demonstrate its utility in detecting the SARS-CoV-2 N ge
Externí odkaz:
https://doaj.org/article/74b9926452694c26addf7fb0b414e93e