Zobrazeno 1 - 10
of 48
pro vyhledávání: '"Hiroshi Motai"'
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 61:1596-1600
The ggt encoding gamma-glutamyltranspeptidase (GGT) from Bacillus subtilis (natto) was cloned and sequenced. The DNA sequence contains a single open reading frame of 1761 bp that might be translated to a protein of 587 amino acid residues, and indica
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 60:255-258
γ-Polyglutamic acid (γ-PGA) produced by Bacillus suhtilis (natto) was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and basic dye staining. Using this method, the molecular weight of γ-PGA was estimated at 275,000. This value was a
Publikováno v:
JOURNAL OF THE BREWING SOCIETY OF JAPAN. 88:50-55
Publikováno v:
Journal of Fermentation and Bioengineering. 76:45-48
In order to obtain a high yield of Lentinus edodes mycelium efficiently used as a liquid spawn for Shiitake mushroom production, a continuous culture system was investigated. The productivity of the mycelium (mg of dry weight of mycelium ml−1h−1)
Publikováno v:
Agricultural and Biological Chemistry. 55:2971-2977
To understand the mechanism by which γ-poly glutamic acid (γ-PGA) in the sticky material of natto was synthesized, we purified the γ-glutamyltranspeptidase (γ-GTP) (EC 2.3.2.2) from the culture broth of Bacillus subtilis (natto) to homogeneity.
Autor:
Hiroshi Motai, Yutaka Ishida, Hiroki Tatsumi, Eiichi Nakano, Atsushi Masaki, Hirofumi Arimura, Haruhide Kawabe, Seiji Murakami, Kohji Murakami
Publikováno v:
Agricultural and Biological Chemistry. 55:2807-2811
The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and con
Autor:
Hiroki Tatsumi, Ryohei Tsuji, Atusi Masaki, Kohji Murakami, Eiichi Nakano, Haruhide Kawabe, Hiroshi Motai, Hirofumi Arimura, Seiji Murakami, Yutaka Ishida
Publikováno v:
Molecular and General Genetics MGG. 228:97-103
The neutral protease II (NpII) from Aspergillus oryzae is a zinc-containing metalloprotease with some unique properties. To elucidate its structure, we isolated a full-length cDNA clone for NpII. Sequence analysis reveals that NpII has a prepro regio
Publikováno v:
Process Biochemistry. 26:39-45
Continuous fermentation of soy sauce with immobilized glutaminase and immobilized cells of Pediococcus halophilus, Zygosaccharomyces rouxii , and Candida versatilis was investigated. Glutamic acid production and both lactic acid and alcohol fermentat
Publikováno v:
Applied Microbiology and Biotechnology. 34:586-590
The effect of soy sauce oil and various other oils on protease production by Aspergillus oryzae NISL 1913 was studied in chemostat cultures (dilution rate=0.02 h−1). Soy sauce oil was consumed as a carbon source by the cells and also accelerated pr
Publikováno v:
Agricultural and Biological Chemistry. 55:2971-2977
To understand the mechanism by which gamma-polyglutamic acid (gamma-PGA) in the sticky material of natto was synthesized, we purified the gamma-glutamyltranspeptidase (gamma-GTP) (EC 2.3.2.2) from the culture broth of Bacillus subtilis (natto) to hom