Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Hiroshi Jinmei"'
Publikováno v:
International Journal of Mass Spectrometry. 333:8-14
Glycoproteomics involving the analysis of glycopeptides by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a new and attractive technique. However, quantitative performance in MALDI-MS is hampered by its poor reproducibili
Autor:
Takashi Nishikaze, Sugawara Shuichi, Kenji Osumi, Ichiro Sugimoto, Masaya Fujita, Fumio Tougasaki, Mamoru Mizuno, Hiroshi Jinmei, Junko Amano
Publikováno v:
Analytical Chemistry. 82:8738-8743
Glycoproteomics holds the promise of new advances in medical technology. However, mass spectrometry has limitations for the structural determination of glycosylated peptides because the hydrophilic nature of the oligosaccharide moiety in glycopeptide
Autor:
Hiroshi Jinmei, Kyoko Aikawa, Hiroshi Abe, Kazuhiro Furukawa, Isamu Matsumoto, Naoko Abe, Satoshi Tsuneda, Atsushi Uchiyama, Yoshihiro Ito, Yuko Kondo
Publikováno v:
Bioconjugate Chemistry. 19:327-333
Enzymatic ligation methods are useful in the diagnostic detection of DNA sequences. Here, we describe the investigation of nonenzymatic phosphorothioate--iodoacetyl DNA chemical ligation as a method for the detection and identification of RNA and DNA
Autor:
Hiroshi Jinmei, Ichiro Sugimoto, Takashi Nishikaze, Toshio Nakamura, Junko Amano, Fumio Tougasaki
Publikováno v:
Journal of Glycomics & Lipidomics.
Glycan is one of the major information-rich biomolecules. Alterations in N-glycans can be associated with many diseases including cancer, and are often observed in the serum of affected patients. As an example, prostate-specific antigen (PSA) is a gl
Publikováno v:
Mass spectrometry (Tokyo, Japan). 1(1)
A standard dried-droplet preparation using 2,5-dihydroxybenzoic acid (2,5-DHBA) as the matrix results in a large variation in signal intensity and poor shot-to-shot reproducibility in matrix-assisted laser desorption/ionization (MALDI). We expected t
Publikováno v:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 879(17-18)
Here, we propose a novel method for the discrimination of α2,3- and α2,6-sialylation on glycopeptides. To stabilize the sialic acids, the carboxyl moiety on the sialic acid as well as the C-terminus and side chain of the peptide backbone were deriv
Publikováno v:
Bioorganicmedicinal chemistry letters. 20(9)
A photoresponsive RNA aptamer to hemin was selected in vitro from a random sequence library of RNAs with azobenzene residues. The aptamer bound to hemin under visible light irradiation and was released by ultraviolet light.
Publikováno v:
Nucleosides, nucleotidesnucleic acids. 22(5-8)
In order to clarify the effect of the base moiety of nucleotide analogs on telomerase inhibition, triphosphate derivatives of biologically active nucleosides, 3′-azido-3′-deoxythymidine (AZT), 2′-deoxy-2′-fluoroarafuranosylthymine (FaraT), ac
Publikováno v:
Chemistry Letters. 37:102-103
RNA aminoacylation, based on an adenosine transfer mechanism, was achieved by the intermolecular transfer of phenylalaninyl adenosine from a donor probe to an acceptor RNA. This method can be applied to a wide variety of unnatural amino acids for tRN
Publikováno v:
Nucleic Acids Symposium Series. 51:365-366
We synthesized two oligonucleotides: one is DNA conjugated with adenosine derivative at 5' terminus as a donor, another RNA phosphorothioated at 3' terminus as an acceptor. By hybridization of the two oligonuleotides, the adenosine derivative was tra