Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Hiroko Osakada"'
Autor:
Tokuko Haraguchi, Takako Koujin, Tomoko Shindo, Şükriye Bilir, Hiroko Osakada, Kohei Nishimura, Yasuhiro Hirano, Haruhiko Asakawa, Chie Mori, Shouhei Kobayashi, Yasushi Okada, Yuji Chikashige, Tatsuo Fukagawa, Shinsuke Shibata, Yasushi Hiraoka
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-15 (2022)
Haraguchi et al. investigate how transfected DNA is incorporated into the nucleus using light and electron microscopy, and the LacI/LacO system. The authors report that cytoplasmic localised plasmid DNA is incorporated into the nucleus as it reforms
Externí odkaz:
https://doaj.org/article/75fe95bd31b84270804da1aa0251b1de
Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival
Publikováno v:
Biology Open, Vol 6, Iss 2, Pp 217-222 (2017)
Upon nitrogen starvation, the fission yeast Schizosaccharomyces pombe forms dormant spores; however, the mechanisms by which a spore sustains life without access to exogenous nutrients remain unclear. Lipid droplets are reservoirs of neutral lipids t
Externí odkaz:
https://doaj.org/article/7f0fbfe7413c44f2af3ee483ff1520be
Autor:
Haruhiko Asakawa, Tomoko Kojidani, Hui-Ju Yang, Chizuru Ohtsuki, Hiroko Osakada, Atsushi Matsuda, Masaaki Iwamoto, Yuji Chikashige, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi
Publikováno v:
PLoS Genetics, Vol 15, Iss 6, p e1008061 (2019)
The nuclear pore complex (NPC) forms a gateway for nucleocytoplasmic transport. The outer ring protein complex of the NPC (the Nup107-160 subcomplex in humans) is a key component for building the NPC. Nup107-160 subcomplexes are believed to be symmet
Externí odkaz:
https://doaj.org/article/15cba3af964a4a68b0c650ec4ed36c2c
Autor:
Go Itoh, Masanori Ikeda, Kenji Iemura, Mohammed Abdullahel Amin, Sei Kuriyama, Masamitsu Tanaka, Natsuki Mizuno, Hiroko Osakada, Tokuko Haraguchi, Kozo Tanaka
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-1 (2018)
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Externí odkaz:
https://doaj.org/article/a688b48b56fc49d089ac16f05d893526
Autor:
Hiroko Osakada, Toyoshi Fujimoto
Publikováno v:
Microscopy (Oxford, England).
Sodium dodecyl sulfate–treated freeze-fracture replica labeling (SDS-FRL) is an electron microscopic (EM) method that can define the two-dimensional distribution of membrane proteins and lipids in a quantitative manner. Despite its unsurpassed meri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a6c6ceca5f3596d6308adbdcb00ff75
https://pubmed.ncbi.nlm.nih.gov/36181465
https://pubmed.ncbi.nlm.nih.gov/36181465
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2502
Tetrahymena is a fascinating organism for studying the nuclear pore complex because it has two structurally and functionally distinct nuclei (macronucleus and micronucleus) within a cell, and there are two compositionally distinct nuclear pore comple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::da08b0365535fa7be793410fa887a4c4
https://pubmed.ncbi.nlm.nih.gov/35412257
https://pubmed.ncbi.nlm.nih.gov/35412257
Autor:
Hiroko Osakada, Yasuha Kinugasa, Yasuhiro Hirano, Tokuko Haraguchi, Tomoko Shindo, Shinsuke Shibata, Yoshino Kubota, Yasushi Hiraoka
Publikováno v:
Communications Biology, Vol 3, Iss 1, Pp 1-14 (2020)
Communications Biology
Communications Biology
The nuclear envelope (NE) continues to the endoplasmic reticulum (ER). Proper partitioning of NE and ER is crucial for cellular activity, but the key factors maintaining the boundary between NE and ER remain to be elucidated. Here we show that the co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5e5946789b605e72154b80be6e40823
http://link.springer.com/article/10.1038/s42003-020-0999-9
http://link.springer.com/article/10.1038/s42003-020-0999-9
Publikováno v:
Methods in Molecular Biology ISBN: 9781071623367
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7b1757ebdd5bc048096f09c2c7756c85
https://doi.org/10.1007/978-1-0716-2337-4_30
https://doi.org/10.1007/978-1-0716-2337-4_30
Autor:
Tatsuo Fukagawa, Tokuko Haraguchi, Yasushi Hiraoka, Yuji Chikashige, Yasuhiro Hirano, Hiroko Osakada, Shouhei Kobayashi, Yasushi Okada, Tomoko Shindo, Shinsuke Shibata, Sukriye Bilir, Haruhiko Asakawa, Takako Koujin, Chie Mori, Kohei Nishimura
Publikováno v:
Communications Biology
Communications Biology, Vol 5, Iss 1, Pp 1-15 (2022)
Communications Biology, Vol 5, Iss 1, Pp 1-15 (2022)
DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe transfection tools. However, they have no effect on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc73eb917b150e04c18a4ee6a74bf502
https://pubmed.ncbi.nlm.nih.gov/35058555
https://pubmed.ncbi.nlm.nih.gov/35058555
Autor:
Tokuko Haraguchi, Yasushi Hiraoka, Sukriye Bilir, Chie Mori, Shouhei Kobayashi, Takako Koujin, Hiroko Osakada, Tomoko Kojidani
Publikováno v:
Genes to Cells. 24:338-353
Reassembly of the nuclear pore complex (NPC) at the end of mitosis is an important event for eukaryotic nuclear function. In this study, we examined the dynamic behaviors of the endoplasmic reticulum (ER) by "Live CLEM" imaging. In metaphase, numerou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b01b52e071f91f8534ec18433b22f3b4
https://doi.org/10.1111/gtc.12677
https://doi.org/10.1111/gtc.12677