Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Hiroki, Kuyama"'
Autor:
Hiroki Kuyama, Tetsuo Ohashi
Publikováno v:
Analytical Biochemistry. 611:113932
An observation that magnetic particles are transported through organogel encouraged us to investigate its feasibility of liquid-phase displacement in DNA extraction using magnetic particles. Organogel for this study was prepared from a gelator, 12-hy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12abe40f6c5d8fa04d14e72736c5f915
https://doi.org/10.1016/j.ab.2020.113932
https://doi.org/10.1016/j.ab.2020.113932
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 112:116-125
Human basic fetoprotein (BFP), found in fetal serum and tissue extracts as well as in extracts of various cancer tissues, has long been known as a marker protein for cancers; however, the primary sequence has not yet been reported. This paper describ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d126c69ed76f7de12bb60530c3d766c7
https://doi.org/10.1016/j.jpba.2015.04.032
https://doi.org/10.1016/j.jpba.2015.04.032
Autor:
Kaoru Kaneshiro, Koichi Tanaka, Kazuya Terasawa, Chikako Hamana, Hiroki Kuyama, Chihiro Nakajima
Publikováno v:
International Journal of Mass Spectrometry. 373:81-87
N- and C-terminal amino acid sequences of human epidermal growth factor receptor-2 (HER2), purified from breast cancer cell lines (SK-BR-3 and BT-474), were analyzed at the protein level using mass spectrometry. Two N-terminal peptides and one C-term
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::557ef3bfd96fd9e39901823c43e862c6
https://doi.org/10.1016/j.ijms.2014.09.010
https://doi.org/10.1016/j.ijms.2014.09.010
Publikováno v:
Journal of Mass Spectrometry. 48:1217-1223
We describe here an optimization study of the sample preparation conditions for sensitive detection of peptides by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Among many factors in the conditions, we varied the percent a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac3dad30b84959055f70383dfb9e3103
https://doi.org/10.1002/jms.3283
https://doi.org/10.1002/jms.3283
Publikováno v:
Journal of Mass Spectrometry. 48:951-960
This paper describes an improved method for the sequence analysis of Arg-containing glycopeptide by MALDI mass spectrometry (MS). The method uses amino group derivatization (4-aza-6-(2,6-dimethyl-1-piperidinyl)-5-oxohexanoic acid N-succinimidyl ester
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::486beb74793eb58fedef3b682d529df0
https://doi.org/10.1002/jms.3241
https://doi.org/10.1002/jms.3241
Autor:
Masashi Tonomoto, Kyosuke Momoji, Shota Sakai, Takashi Hirata, Hiroki Kuyama, Taro Masuda, Osamu Nishimura, Ryosuke Otomo, Tatsuya Sugawara
Publikováno v:
Fish & Shellfish Immunology. 32:61-68
Melanization is one of the major immune responses in arthropods. Prophenoloxidases (proPOs) catalyze the oxidation of mono- or o-diphenols, a reaction that is the key initial step of melanin formation. Well-characterized proPOs from crustaceans are s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7c36880dbe33e205b2cf76284164472
https://doi.org/10.1016/j.fsi.2011.10.020
https://doi.org/10.1016/j.fsi.2011.10.020
Autor:
Kazuhiro Sonomura, Ei-ichi Matsuo, Susumu Tsunasawa, Shiroh Futaki, Hiroki Kuyama, Osamu Nishimura
Publikováno v:
Analytical Biochemistry. 410:214-223
Many eukaryotic proteins are blocked at the α-amino group of their N-terminal with various modifications, thereby making it difficult to determine their N-terminal sequence by protein sequencer. We propose a novel method for selectively isolating th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ac0b5685da8424bbcc0963ca8dcbd20
https://doi.org/10.1016/j.ab.2010.12.006
https://doi.org/10.1016/j.ab.2010.12.006
Autor:
Kazumasa Sakurai, Hironobu Naiki, Hisashi Yagi, Yuji Goto, Daisaku Ozawa, Toru Kawakami, Osamu Nishimura, Hiroki Kuyama, Toshinori Shimanouchi, Ryoichi Kuboi
Publikováno v:
Journal of Biological Chemistry. 285(25):19660-19667
This research was originally published in the Journal of Biological Chemistry. Hisashi Yagi, Daisaku Ozawa, Kazumasa Sakurai, Toru Kawakami, Hiroki Kuyama, Osamu Nishimura,Toshinori Shimanouchi, Ryoichi Kuboi, Hironobu Naiki, and Yuji Goto. Laser-ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f0c8e321c8771adc32d83c32b619d9c
https://hdl.handle.net/11094/71286
https://hdl.handle.net/11094/71286
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 19:6544-6547
A novel method for selectively labeling and isolating N-terminal peptide from protein has been developed. An N(alpha)-amino group of protein was converted to a carbonyl group through transamination reaction and the resulting carbonyl group was modifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::619b7a96f04fe15a46ef1f05b75356ba
https://doi.org/10.1016/j.bmcl.2009.10.044
https://doi.org/10.1016/j.bmcl.2009.10.044
Publikováno v:
PROTEOMICS. 9:4063-4070
We describe a mass spectrometric method for distinguishing between free and modified forms of the C-terminal carboxyl group of peptides and proteins, in combination with chemical approaches for the isolation of C-terminal peptides and site-specific d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb01d34f9b9f0bdda0d8c4013b808de5
https://doi.org/10.1002/pmic.200900267
https://doi.org/10.1002/pmic.200900267