Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Hiroji Yoshikawa"'
Autor:
Fumio Fukui, Katsuya Gomi, Yoichiro Shiba, Nobufusa Serizawa, Hiroji Yoshikawa, Chiho Ono, Ichiro Watanabe
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 65:94-101
Phospholipase A1 (PLA1) is a hydrolytic enzyme that catalyzes the removal of the acyl group from position 1 of lecithin to form lysolecithin. The PLA1 gene, which had been cloned from Aspergillus oryzae, was expressed in Saccharomyces cerevisiae and
Publikováno v:
Journal of Bioscience and Bioengineering. 89:426-430
In the course of studying carboxypeptidase Y (CPY) production, we found that the expression level of the gene, which is under the control of the GAL10 promoter, increased in a Saccharomyces cerevisiae gal80 mutant grown in a medium containing ethanol
Autor:
Hiroji Yoshikawa, Yoshio Yao, Hidetsune Tamaoki, Fumio Fukui, Kawaguchi Junko, Hirokazu Ishikawa, Takeshi Ono
Publikováno v:
Journal of Bioscience and Bioengineering. 87:296-301
In order to develop a large-scale, high-yield production process for human calcitonin (hCT) in Escherichia coli , a stable expression plasmid was constructed and the expressed protein was modified for efficient cleavage by protease. Multiple copies o
Autor:
Takao Okazaki, Ryuzo Enokita, Koichi Nonaka, Takahiro Yamamoto, Hiroji Yoshikawa, Fumio Maruyama
Publikováno v:
Actinomycetologica. 13:32-41
In the production culture of milbemycin α11 and α14 by Streptomyces hygroscopicus subsp. aureolacrimosus SANK 60393 (strain MA3-6126), two types of morphological mutants appeared in the late production phase, in which the milbemycin production rate
Publikováno v:
Journal of Fermentation and Bioengineering. 86:545-549
Carboxypeptidase Y (CPY; EC 3. 4. 16. 1) encoded by the PRC1 gene is a yeast vacuolar protease. It enters the vacuole as a zymogen, proCPY, which is activated by vacuolar enzymes, proteinase A (PrA) and proteinase B (PrB). We previously showed that a
Publikováno v:
Journal of Pesticide Science. 19:S125-S131
Publikováno v:
Biotechnology and Bioengineering. 42:815-820
An automatic feeding process for microbial hydroxylation of ML236B sodium salt (ML-236B Na; compactin) by Streptomyces carbophilus SANK 62585 was developed. The hydroxylated product, pravastatin sodium salt (pravastatin; trade name Mevalotin), is an
Publikováno v:
Journal of Fermentation and Bioengineering. 76:482-486
Fuzzy control was applied to the fermentation of ML-236B by Penicillium citrinum. In order to maintain the pH of the culture at a suitable level for ML-236B production (pH 3.7 to 3.9), a computer aided system using fuzzy control was designed for cont
Publikováno v:
Journal of Fermentation and Bioengineering. 76:476-481
In order to scale-up the production of ML-236B, a precursor of pravastatin sodium, by Penicillium citrinum H strain, a method for controlling mycelial morphology was developed to increase the number of free mycelia in the preculture process. The use
Autor:
Hiroji Yoshikawa, Tetsuya Shioiri, Masatoshi Arai, Masahiko Hosobuchi, Jo Ohyama, Seigo Iwado
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 57:1414-1419
In order to increase the yield of ML-236B, an intermediate for pravastatin Na (an inhibitor of cholesterol synthesis) production, the improvements of an ML-236B producing strain of Penicillium citrinum, the medium composition, and the culture conditi