Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Hirohito Haruki"'
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e37598 (2012)
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in ce
Externí odkaz:
https://doaj.org/article/259dfdcff39a4f15aa49653ae0a2040b
Autor:
Christopher Chidley, Hirohito Haruki, Miriam Grønlund Pedersen, Cindy Fellay, Simone Moser, Kai Johnsson
Publikováno v:
CHIMIA, Vol 65, Iss 9 (2011)
The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reve
Externí odkaz:
https://doaj.org/article/b976ac4167f84543ae7cef4d8f800546
Publikováno v:
Journal of Biological Chemistry
Tryptophan metabolites in the kynurenine pathway are up-regulated by pro-inflammatory cytokines or glucocorticoids, and are linked to anti-inflammatory and immunosuppressive activities. In addition, they are up-regulated in pathologies such as cancer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7511fa30c83235522371cdc7f5a84d5
https://europepmc.org/articles/PMC4705385/
https://europepmc.org/articles/PMC4705385/
Publikováno v:
Nature Chemica Biology
We introduce an approach for detection of drug-protein interactions that combines a new yeast three-hybrid screening for identification of interactions with affinity chromatography for their unambiguous validation. We applied the methodology to the p
Publikováno v:
FEBS letters. 581(17):3283-3288
application/pdf
We identified nucleophosmin/B23 as a component of template-activating factor-III that stimulates the DNA replication from the adenovirus DNA complexed with viral basic core proteins. Here, we have studied the functional interacti
We identified nucleophosmin/B23 as a component of template-activating factor-III that stimulates the DNA replication from the adenovirus DNA complexed with viral basic core proteins. Here, we have studied the functional interacti
Publikováno v:
Biochemistry. 45:303-313
Template Activating Factor (TAF) I remodels the adenovirus (Ad) core structure composed of the Ad genome DNA and basic viral core proteins and stimulates in vitro DNA replication and transcription of the Ad core. We have recently reported that TAF-I
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e37598 (2012)
PLoS ONE
PLoS ONE
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in ce
Autor:
Cindy Fellay, Simone Moser, Miriam Grønlund Pedersen, Christopher Chidley, Hirohito Haruki, Kai Johnsson
Publikováno v:
CHIMIA, Vol 65, Iss 9 (2011)
The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reve
Publikováno v:
Nucleic Acids Research
The adenovirus genome forms chromatin-like structure with viral core proteins. This complex supports only a low level of transcription in a cell-free system, and thus core proteins have been thought to be negative factors for transcription. The mecha
Publikováno v:
Molecular cell. 31(6)
The anchor-away (AA) technique depletes the nucleus of Saccharomyces cerevisiae of a protein of interest (the target) by conditional tethering to an abundant cytoplasmic protein (the anchor) by appropriate gene tagging and rapamycin-dependent heterod