Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Hirofumi Kakihara"'
Autor:
Hiroki Tanemura, Ryunosuke Kitamura, Yasuko Yamada, Masato Hoshino, Hirofumi Kakihara, Koichi Nonaka
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-13 (2023)
Abstract Chinese hamster ovary (CHO) cells are widely utilized in the production of antibody drugs. To ensure the production of large quantities of antibodies that meet the required specifications, it is crucial to monitor and control the levels of m
Externí odkaz:
https://doaj.org/article/fde8569c3ba64d8ab27e4990f206e264
Autor:
Hiroki Tanemura, Kenji Masuda, Takeshi Okumura, Eri Takagi, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka, Ryo Ushioda
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract Chinese hamster ovary (CHO) cells are widely used for manufacturing antibody drugs. We attempted to clone a novel high-expression promoter for producing monoclonal antibodies (mAbs) based on transcriptome analysis to enhance the transcriptio
Externí odkaz:
https://doaj.org/article/fcda47d8d6944ae2a31afe14caac6a4e
Publikováno v:
PLoS ONE, Vol 16, Iss 4, p e0250416 (2021)
There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to robu
Externí odkaz:
https://doaj.org/article/81f967fa3b6a41ac92e77c8158a4846e
Autor:
Diana Kreitmayer, Srikanth R. Gopireddy, Tomomi Matsuura, Yuichi Aki, Yuta Katayama, Taihei Sawada, Hirofumi Kakihara, Koichi Nonaka, Thomas Profitlich, Nora A. Urbanetz, Eva Gutheil
Publikováno v:
Bioengineering, Vol 9, Iss 5, p 206 (2022)
Two-way Euler-Lagrange simulations are performed to characterize the hydrodynamics in the single-use bioreactor Mobius® CellReady 3 L. The hydrodynamics in stirred tank bioreactors are frequently modeled with the Euler–Euler approach, which cannot
Externí odkaz:
https://doaj.org/article/6aa4572d7b5e46ac9d185bebb6862002
Autor:
Diana Kreitmayer, Srikanth R. Gopireddy, Tomomi Matsuura, Yuichi Aki, Yuta Katayama, Takuya Nakano, Takuma Eguchi, Hirofumi Kakihara, Koichi Nonaka, Thomas Profitlich, Nora A. Urbanetz, Eva Gutheil
Publikováno v:
Bioengineering, Vol 9, Iss 1, p 22 (2022)
Understanding the hydrodynamic conditions in bioreactors is of utmost importance for the selection of operating conditions during cell culture process development. In the present study, the two-phase flow in the lab-scale single-use bioreactor Xcelle
Externí odkaz:
https://doaj.org/article/a100df29751f42e1a98857e954c528c8
Autor:
Koichi Nonaka, Srikanth R. Gopireddy, Eva Gutheil, Hirofumi Kakihara, Nora Anne Urbanetz, Tomomi Matsuura, Diana Kreitmayer, Shizuka Kondo
Publikováno v:
World Congress on Momentum, Heat and Mass Transfer.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1f86d9bf5729f3336e0f9118278d837c
https://doi.org/10.11159/icmfht20.185
https://doi.org/10.11159/icmfht20.185
Autor:
Hirofumi Kakihara, Srikanth R. Gopireddy, Yuta Katayama, Thomas Profitlich, Tomomi Matsuura, Koichi Nonaka, Eva Gutheil, Diana Kreitmayer, Nora Anne Urbanetz, Yuichi Aki
Publikováno v:
Biochemical Engineering Journal. 177:108237
The optimization of operating conditions is vital for cell culture process development. In the present study, the pilot-scale single-use bioreactor XcellerexTM XDR-200, which is typically used in cell culture scale-up, is investigated. This unbaffled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0cce938b3ff52667531b117c9bb5a3b0
https://doi.org/10.1016/j.bej.2021.108237
https://doi.org/10.1016/j.bej.2021.108237
Publikováno v:
PLoS ONE, Vol 16, Iss 4, p e0250416 (2021)
PLoS ONE
PLoS ONE
There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to robu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1ffdb987e488f7b00e086ae047c7221
https://doi.org/10.1371/journal.pone.0250416
https://doi.org/10.1371/journal.pone.0250416
Autor:
Hiroaki Inoue, Hirofumi Kakihara, Masahiro Takagi, Masao Kitabayashi, Bunsei Tsuruga-shi Kawakami, Tadayuki Imanaka, Masanori Oka, Motomu Nishioka
Publikováno v:
Applied and Environmental Microbiology. 63:4504-4510
The DNA polymerase gene from the archaeon Pyrococcus sp. strain KOD1 (KOD DNA polymerase) contains a long open reading frame of 5,013 bases that encodes 1,671 amino acid residues (GenBank accession no. D29671). Similarity analysis revealed that the D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4d37bbe9c1d5e068d359a344215ac7c
https://doi.org/10.1128/aem.63.11.4504-4510.1997
https://doi.org/10.1128/aem.63.11.4504-4510.1997