Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Hilary L Ashe"'
Autor:
Lauren Forbes Beadle, Jennifer C Love, Yuliya Shapovalova, Artem Artemev, Magnus Rattray, Hilary L Ashe
Publikováno v:
PLoS Biology, Vol 21, Iss 1, p e3001956 (2023)
Regulation of mRNA degradation is critical for a diverse array of cellular processes and developmental cell fate decisions. Many methods for determining mRNA half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use a non-inv
Externí odkaz:
https://doaj.org/article/da389f194bd547d28a6e4596d4a3ccd0
Autor:
Lisa Deignan, Marco T Pinheiro, Catherine Sutcliffe, Abbie Saunders, Scott G Wilcockson, Leo A H Zeef, Ian J Donaldson, Hilary L Ashe
Publikováno v:
PLoS Genetics, Vol 12, Iss 7, p e1006164 (2016)
The BMP signaling pathway has a conserved role in dorsal-ventral axis patterning during embryonic development. In Drosophila, graded BMP signaling is transduced by the Mad transcription factor and opposed by the Brinker repressor. In this study, usin
Externí odkaz:
https://doaj.org/article/bed30bb8962a4b00b74ed4bb17fea2df
Publikováno v:
eLife, Vol 4 (2015)
Members of the Tolloid family of metalloproteinases liberate BMPs from inhibitory complexes to regulate BMP gradient formation during embryonic dorsal-ventral axis patterning. Here, we determine mechanistically how Tolloid activity is regulated by it
Externí odkaz:
https://doaj.org/article/c22e894d32e746c6b16452286777a735
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100812- (2021)
Summary: Significant regulation of gene expression is mediated at the translation level. Here, we describe protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living Drosophila embryos. These protocols use th
Externí odkaz:
https://doaj.org/article/58675458936343888e7262d75d25ccd8
Autor:
Caroline Hoppe, Hilary L. Ashe
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100379- (2021)
Summary: Visualizing transcription live in Drosophila is providing important new insights into the spatiotemporal regulation of transcription. Here, we describe a protocol to visualize and quantitate transcription from gene loci that are tagged with
Externí odkaz:
https://doaj.org/article/ddd55a296ce1432db863a21034d39698
Autor:
Scott G. Wilcockson, Hilary L. Ashe
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100371- (2021)
Summary: The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the Drosophila ovarian germli
Externí odkaz:
https://doaj.org/article/ad26b1a1ffcf42d5bc882fec7adf7fd8
Autor:
Caroline Hoppe, Hilary L. Ashe
Publikováno v:
STAR Protocols, Vol 2, Iss 1, Pp 100380- (2021)
Summary: CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosoph
Externí odkaz:
https://doaj.org/article/68c80ef47072438c825250851d933573
Publikováno v:
Cell Reports. 42:112382
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::50c71ef3c3c3f7b6a9fb1b862704e141
https://doi.org/10.1016/j.celrep.2023.112382
https://doi.org/10.1016/j.celrep.2023.112382
A BMP gradient is essential for patterning the dorsal-ventral axis of invertebrate and vertebrate embryos. The extracellular BMP binding protein Short Gastrulation (Sog) in Drosophila plays a key role in BMP gradient formation. In this study, we comb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439b5c8a19b1fc1863f190bf3079576d
https://doi.org/10.1101/2022.05.13.491850
https://doi.org/10.1101/2022.05.13.491850
Autor:
Lauren Forbes Beadle, Jennifer C. Love, Yuliya Shapovalova, Artem Artemev, Magnus Rattray, Hilary L. Ashe
Regulation of mRNA degradation is critical for a diverse array of cellular processes and developmental cell fate decisions. Many methods for determining mRNA half-lives rely on transcriptional inhibition or metabolic labelling. Here we use a non-inva
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::73a05e39e5878c51d647ff4cb3041ad4
https://doi.org/10.1101/2022.03.17.484585
https://doi.org/10.1101/2022.03.17.484585