Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Hideyuki Shimaoka"'
Autor:
Hiroyuki Kuwano, Toyo Nishimura, Hideyuki Shimaoka, Abby R. Saniabadi, Takashi Hayashi, Takayuki Asao, Shin Yazawa
Publikováno v:
BioMed Research International, Vol 2013 (2013)
BioMed Research International
BioMed Research International
Human plasmaα1-acid glycoprotein (AGP) from cancer patients and healthy volunteers was purified by sequential application of ion-exchange columns, and N-linked glycans enzymatically released from AGP were labeled and applied to a mass spectrometer.
Autor:
Yuichi Yoshimura, Yoshihiro Natori, Erina Hayashi, Atsushi Kato, Hiroki Takahata, Saori Miyauchi, Robert J. Nash, Jun Koseki, Izumi Nakagome, Hideyuki Shimaoka, Isao Adachi, Tatsushi Imahori, Shuichi Hirono
Publikováno v:
Journal of Medicinal Chemistry. 55:10347-10362
We report on the synthesis and the biological evaluation of a series of α-1-C-alkylated 1,4-dideoxy-1,4-imino-l-arabinitol (LAB) derivatives. The asymmetric synthesis of the derivatives was achieved by asymmetric allylic alkylation, ring-closing met
Publikováno v:
Polymer Journal. 44:269-277
Intact glycans were recovered and labeled with common fluorescent dyes by using polymer beads possessing a high density of hydrazide groups. To achieve that, the method for releasing glycans from the beads was examined and optimized. Additionally, fu
Autor:
Maho Amano, Hideyuki Shimaoka, Shota Takimori, Yasuro Shinohara, Imre Kacskovics, Lennart Hammarström, Naoki Fujitani, Shin-Ichiro Nishimura, Yasuhiro Takegawa, Jun-ichi Furukawa, Tadashi Yamashita
Publikováno v:
FEBS Journal. 278:3769-3781
Milk provides nutritional, immunological and developmental components for newborns. Whereas identification of such components has been performed by targeting proteins and free oligosaccharides, structural and functional analyses of the N-glycome of m
Autor:
Shin-Ichiro Nishimura, Mika Nakano, Yoshiaki Miura, Masaki Kurogochi, Yasuro Shinohara, Hiromitsu Kuramoto, Jun-ichi Furukawa, Hideyuki Shimaoka
Publikováno v:
Analytical Chemistry. 80:1094-1101
Changes in protein glycosylation profoundly affect protein function. To understand these effects of altered protein glycosylation, we urgently need high-throughput technologies to analyze glycan expression and glycan-protein interactions. Methods are
Autor:
Tomohiro Onodera, Norimasa Iwasaki, Kuniharu Ijiro, Kenichi Niikura, Hideyuki Shimaoka, Ryosuke Kamitani
Publikováno v:
Bulletin of the Chemical Society of Japan. 80:1808-1813
We describe a graft-type modification of a solid surface to be able to immobilize unprotected carbohydrates with a desired pattern. Water soluble carbohydrate-trapping polymers were grown from a so...
Specific Cell Behavior of Human Fibroblast onto Carbohydrate Surface Detected by Glycoblotting Films
Autor:
Ryusuke Kamitani, Norimasa Iwasaki, Noriko Nagahori, Akio Minami, Hideyuki Shimaoka, Tomohiro Onodera, Tokifumi Majima, Kenichi Niikura, Shin-Ichiro Nishimura
Publikováno v:
Biomacromolecules. 7:2949-2955
We synthesized an aminooxyl polymer that is reactive with the reduced end of carbohydrates using our sugar-displaying approach. The carbohydrates were easily immobilized on the polymer film (glycoblotting film) by simple immersion in a in sugar solut
Autor:
Shota, Takimori, Hideyuki, Shimaoka, Jun-Ichi, Furukawa, Tadashi, Yamashita, Maho, Amano, Naoki, Fujitani, Yasuhiro, Takegawa, Lennart, Hammarström, Imre, Kacskovics, Yasuro, Shinohara, Shin-Ichiro, Nishimura
Publikováno v:
The FEBS journal. 278(19)
Milk provides nutritional, immunological and developmental components for newborns. Whereas identification of such components has been performed by targeting proteins and free oligosaccharides, structural and functional analyses of the N-glycome of m
Autor:
Shin-Ichiro Nishimura, Satoru Todo, Masaki Kurogochi, Yoshiaki Miura, Megumi Hato, Jun-ichi Furukawa, Michitaka Ozaki, Hideyuki Shimaoka, Mitsuhiro Tada, Hiromitsu Kuramoto, Yasuro Shinohara, Kazuaki Nakanishi
Publikováno v:
Molecularcellular proteomics : MCP. 7(2)
Recent progress in mass spectrometry has led to new challenges in glycomics, including the development of rapid glycan enrichment techniques. A facile technique for exploration of a carbohydrate-related biomarker is important because proteomics resea
Autor:
Jun-ichi Furukawa, Masaki Kurogochi, Hideyuki Shimaoka, Hiroshi Hinou, Yasuro Shinohara, Hiromitsu Kuramoto, Shin-Ichiro Nishimura, Yoshiaki Miura, Yoko Kita
Publikováno v:
Chemistry (Weinheim an der Bergstrasse, Germany). 13(6)
The development of rapid and efficient methods for high-throughput protein glycomics is of growing importance because the glycoform-focused reverse proteomics/genomics strategy will greatly contribute to the discovery of novel biomarkers closely rela