Zobrazeno 1 - 10
of 367
pro vyhledávání: '"Hidetoshi Kotera"'
Autor:
Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, Li Liu
Publikováno v:
Stem Cell Reports, Vol 11, Iss 1, Pp 142-156 (2018)
Summary: We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation
Externí odkaz:
https://doaj.org/article/9e3d5f4c5f774994967bc7dbe24f41f7
Autor:
Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku
Publikováno v:
Genome Biology, Vol 19, Iss 1, Pp 1-11 (2018)
Abstract We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA
Externí odkaz:
https://doaj.org/article/a25df1d8930847d780c622981c9a8d5e
Autor:
Junjun Li, Itsunari Minami, Motoko Shiozaki, Leqian Yu, Shin Yajima, Shigeru Miyagawa, Yuji Shiba, Nobuhiro Morone, Satsuki Fukushima, Momoko Yoshioka, Sisi Li, Jing Qiao, Xin Li, Lin Wang, Hidetoshi Kotera, Norio Nakatsuji, Yoshiki Sawa, Yong Chen, Li Liu
Publikováno v:
Stem Cell Reports, Vol 9, Iss 5, Pp 1546-1559 (2017)
Summary: High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature ra
Externí odkaz:
https://doaj.org/article/66b39ba0bb4a435b98ea2d6be99f8a63
Publikováno v:
Micromachines, Vol 11, Iss 6, p 571 (2020)
2D and 3D topographic cues made of photoresist, a polymer, are used for cell culture and cell analysis. Photoresists used for cell analysis provide the surface conditions necessary for proper cell growth, along with patterning properties of a wide ra
Externí odkaz:
https://doaj.org/article/d987814284f3487c83975e7312b66efb
Autor:
Hidetaka UENO, Shoji KOMAI, Kyohei TERAO, Hidekuni TAKAO, Fusao SHIMOKAWA, Hidetoshi KOTERA, Takaaki SUZUKI
Publikováno v:
Mechanical Engineering Journal, Vol 3, Iss 4, Pp 15-00570-15-00570 (2016)
In this paper, we propose a simple plasma-less fabrication and integration method for biomicrodevice applications. For optogenetics, microlenses and pinholes irradiating local sight of biological tissue with focused visible light and electrodes measu
Externí odkaz:
https://doaj.org/article/6d7d90c6f9ae405a89bca3f40916d000
Autor:
Tasuku Nakahara, Junya Suzuki, Yuki Hosokawa, Fusao Shimokawa, Hidetoshi Kotera, Takaaki Suzuki
Publikováno v:
Magnetochemistry, Vol 4, Iss 1, p 7 (2018)
Microvalves play an important role in fluid control in micro total analysis systems (µTAS). Previous studies have reported complex fabrication processes for making microvalve elements in a channel. Hence, there is a need for a simpler microvalve fab
Externí odkaz:
https://doaj.org/article/ac4925326dcc465b9ca4a6fc09fe075a
Publikováno v:
Communications of the ACM. Jul2023, Vol. 66 Issue 7, p94-95. 2p. 1 Diagram.
Autor:
Mahmoud N. Abdelmoez, Hirofumi Shintaku, Ryuji Yokokawa, Hidetoshi Kotera, Yuka Ozaki, Yusuke Oguchi
Publikováno v:
Analytical Chemistry. 92:1485-1492
The physical fractionation of cytoplasmic versus nuclear components of cells is a key step for studying the subcellular localization of molecules. The application of an electric field is an emerging method for subcellular fractionation of proteins an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aebc63f67211dbfa86204d137246f766
https://doi.org/10.1021/acs.analchem.9b04739
https://doi.org/10.1021/acs.analchem.9b04739
Autor:
Hirofumi Shintaku, Tomohiro Nakagawa, Hidetoshi Kotera, Ryuji Yokokawa, Shumpei Ohara, Tamanna Ishrat Farhana
Publikováno v:
Langmuir. 35:13003-13010
Cooperativity of motor proteins is essential for intracellular transport. Although their motion is unidirectional, they often cause bidirectional movement by different types of motors as seen in organelles. However, in vitro assessments of such cellu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3d8526f1aa3ca78bd46764f61c16a69
https://doi.org/10.1021/acs.langmuir.9b01411
https://doi.org/10.1021/acs.langmuir.9b01411
Publikováno v:
Colloids and Surfaces A: Physicochemical and Engineering Aspects. 565:188-194
Cell surface reactions such as material surface characteristics and cell proliferation/adhesion rates are correlated and this correlation can be used as early determination criteria for shortening the development period and reducing costs of selectin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2943afa7e3bf07a48bb29cf36a2a70c
https://doi.org/10.1016/j.colsurfa.2018.12.059
https://doi.org/10.1016/j.colsurfa.2018.12.059