Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Hideto Ueda"'
Publikováno v:
Transgenic Research. 15:229-240
The objective of this study was to examine whether the ICSI-mediated gene transfer method using in vitro matured oocytes and frozen sperm head could actually produce transgenic pigs. We also aimed at examining whether transgenic pigs can be cloned fr
Publikováno v:
Journal of Biomedical Materials Research Part A. :19-27
To obtain a bioartificial kidney composed of a porous polymer membrane and renal cells, a polysulfone (PSf) membrane (PSM) blended with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was prepared. The PSM flat membrane with a porous structure
Publikováno v:
Journal of Reproduction and Development. 51:405-410
Incomplete cytoplasmic maturation of in vitro matured (IVM) oocytes has been known to cause microtubule and microfilament alterations, which may result in abnormal pronuclear formation and failed embryonic development. We examined the influences of m
Autor:
Ritsuko Esaki, Hiroshi Nagashima, Hideto Ueda, Hitoshi Ushijima, Hiroki Yoshioka, Masashige Kuwayama, Ryo Tomii, Kazumasa Hirakawa, Mayuko Kurome
Publikováno v:
Biology of Reproduction. 71:432-437
This study describes a cryopreservation method for porcine in vitro-produced (IVP) embryos using as a model parthenogenetic embryos derived from in vitro-matured (IVM) oocytes. IVP embryos at the expanded blastocyst stage were cryopreserved by vitrif
Autor:
Ryo Tomii, Naohiro Wako, Mayuko Kurome, Koichiro Kano, Takashi Ochiai, Hideto Ueda, Kazumasa Hirakawa, Hiroshi Nagashima
Publikováno v:
Cloning and stem cells. 7(4)
The aim of the present study was to determine whether porcine preadipocytes can be efficient donor cells for somatic cell nuclear transfer (SCNT) in pigs. Primary culture of porcine preadipocytes was established by de-differentiating mature fat cells
Autor:
Kazumasa Hirakawa, Hiroshi Nagashima, K. Hiruma, Ryo Tomii, Hideto Ueda, Mayuko Kurome, S. Ueno, Koji Miyazaki
Publikováno v:
The Journal of reproduction and development. 51(1)
This study aimed to evaluate the quality of porcine somatic cell nuclear transfer (SCNT) embryos by examining its gene transcription patterns. Embryos were produced by SCNT, intracytoplasmic sperm injection (ICSI) or under different conditions, and t
Publikováno v:
Reproduction, Fertility and Development. 17:184
Since the first success in producing cloned pigs, donor cells have been limited to fetal fibroblasts and a few other cell types. The aim of the present study was to determine if porcine preadipocytes can be efficient donor cells for somatic cell nucl
Autor:
F. Endo, S. Matsumoto, Hiroshi Nagashima, Ryo Tomii, Hideto Ueda, K. Nakamura, K. Okumura, Mayuko Kurome
Publikováno v:
Reproduction, Fertility and Development. 17:171
The aim of the present study was to investigate whether a somatic stem cell derived from the salivary gland can be an efficient donor cell for pig cloning. Somatic stem cells (salivary gland progenitor cells, SGP) were isolated from the salivary glan
Autor:
Ryo Tomii, Mayuko Kurome, Takashi Ochiai, Naohiro Wako, Hideto Ueda, Kazumasa Hirakawa, Koichiro Kano, Hiroshi Nagashima
Publikováno v:
Cloning & Stem Cells; Winter2005, Vol. 7 Issue 4, p279-288, 10p