Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Hidetaka Nakata"'
Autor:
Takeshi Hanami, Tetsuya Tanabe, Takuya Hanashi, Mitsushiro Yamaguchi, Hidetaka Nakata, Yasumasa Mitani, Yasumasa Kimura, Takahiro Soma, Kengo Usui, Michiko Isobe, Takashi Ogawa, Masayoshi Itoh, Yoshihide Hayashizaki, Seiji Kondo
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0243319 (2020)
Here, we report a rapid and ultra-sensitive detection technique for fluorescent molecules called scanning single molecular counting (SSMC). The method uses a fluorescence-based digital measurement system to count single molecules in a solution. In th
Externí odkaz:
https://doaj.org/article/d7578cc2ac6a49d2a5f57117370c6106
Autor:
Hidetaka Nakata, Takashi Kanno, Naohito Ohno, Daisuke Yamanaka, Tetsuya Tanabe, Yoshiyuki Adachi, Ken-ichi Ishibashi
Publikováno v:
International Journal of Molecular Sciences, Vol 22, Iss 5977, p 5977 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 11
International Journal of Molecular Sciences
Volume 22
Issue 11
To overcome the limitations of the Limulus amebocyte lysate (LAL) assay method for the diagnosis of invasive fungal infection, we applied a reaction system combining recombinant β-glucan binding proteins and a scanning single-molecule counting (SSMC
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc81833b9e78e8de1946fab203da38a7
https://www.mdpi.com/1422-0067/22/11/5977
https://www.mdpi.com/1422-0067/22/11/5977
Autor:
Michiko Isobe, Masayoshi Itoh, Hidetaka Nakata, Seiji Kondo, Takuya Hanashi, Tetsuya Tanabe, Takeshi Hanami, Yasumasa Mitani, Yasumasa Kimura, Yoshihide Hayashizaki, Takahiro Soma, Takashi Ogawa, Mitsushiro Yamaguchi, Kengo Usui
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0243319 (2020)
PLoS ONE
PLoS ONE
Here, we report a rapid and ultra-sensitive detection technique for fluorescent molecules called scanning single molecular counting (SSMC). The method uses a fluorescence-based digital measurement system to count single molecules in a solution. In th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::067fcda8d76bc2b37aa73f72a1f1807e
https://doaj.org/article/d7578cc2ac6a49d2a5f57117370c6106
https://doaj.org/article/d7578cc2ac6a49d2a5f57117370c6106
Publikováno v:
Analytical Biochemistry. 625:114220
In this paper, we present the applications of our newly developed, highly sensitive fluorescent detection method referred to as scanning single-molecule counting (SSMC). We found that the target RNA added to the total RNA was detected with high sensi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aab2ec78337ad51b4b02c81506ac15b
https://doi.org/10.1016/j.ab.2021.114220
https://doi.org/10.1016/j.ab.2021.114220
Publikováno v:
Journal of Bioscience and Bioengineering. 97:395-399
Factors that affect the efficiency of in vitro synthesis of mutant proteins that contain nonnatural amino acids were investigated. The process of the nonnatural mutagenesis consists of chemical aminoacylation of a tRNA that contains a 4-base anticodo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4a9318227e1c8a0dca53a2d5e7e7a08
https://doi.org/10.1016/s1389-1723(04)70225-x
https://doi.org/10.1016/s1389-1723(04)70225-x
Autor:
Kazutaka Nishikawa, Seiji Kondo, Hidetaka Nakata, Mitsushiro Yamaguchi, Kunio Hori, Tetsuya Tanabe, Takuya Hanashi
Publikováno v:
SPIE Proceedings.
A new, simple technique for single molecule fluorescence detection has been developed and detection limit of less than 100 aM fluorophores has been demonstrated. The technique, similarly to Fluorescence Correlation Spectroscopy (FCS) and other relate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f3ca0f194e4c28239387bf04ef7b488
https://doi.org/10.1117/12.2037645
https://doi.org/10.1117/12.2037645
Publikováno v:
Analytical biochemistry. 390(2)
We developed novel substrates for protease activity evaluation by fluorescence correlation spectroscopy (FCS). Substrates were labeled in a position-specific manner with a fluorophore near the N terminus and included a C-terminal, 30 kDa, highly solu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97164ed4e3e961d7fab4f3a95c0215c2
https://pubmed.ncbi.nlm.nih.gov/19394304
https://pubmed.ncbi.nlm.nih.gov/19394304
Publikováno v:
Analytical biochemistry. 348(2)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a68cdc0e6925600264be0658214ea7d7
https://pubmed.ncbi.nlm.nih.gov/16307719
https://pubmed.ncbi.nlm.nih.gov/16307719