Zobrazeno 1 - 10
of 69
pro vyhledávání: '"Hideki Uosaki"'
PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells
Autor:
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Publikováno v:
Communications Medicine, Vol 3, Iss 1, Pp 1-12 (2023)
Abstract Background Base editing via CRISPR-Cas9 has garnered attention as a method for correcting disease-specific mutations without causing double-strand breaks, thereby avoiding large deletions and translocations in the host chromosome. However, i
Externí odkaz:
https://doaj.org/article/8d87658f0b3149538b80ca60fb8ca67c
Autor:
Chika Watanabe, Hitoshi Osaka, Miyuki Watanabe, Akihiko Miyauchi, Eriko F. Jimbo, Takeshi Tokuyama, Hideki Uosaki, Yoshihito Kishita, Yasushi Okazaki, Takanori Onuki, Tomohiro Ebihara, Kenichi Aizawa, Kei Murayama, Akira Ohtake, Takanori Yamagata
Publikováno v:
Molecular Genetics and Metabolism Reports, Vol 34, Iss , Pp 100951- (2023)
Coenzyme Q10 (CoQ10) is involved in ATP production through electron transfer in the mitochondrial respiratory chain complex. CoQ10 receives electrons from respiratory chain complex I and II to become the reduced form, and then transfers electrons at
Externí odkaz:
https://doaj.org/article/3d16e4198f7d49d399b9dffcfcdfd7dc
Autor:
Takeshi Tokuyama, Hideki Uosaki, Ayumu Sugiura, Gen Nishitai, Keisuke Takeda, Shun Nagashima, Isshin Shiiba, Naoki Ito, Taku Amo, Satoshi Mohri, Akiyuki Nishimura, Motohiro Nishida, Ayumu Konno, Hirokazu Hirai, Satoshi Ishido, Takahiro Yoshizawa, Takayuki Shindo, Shingo Takada, Shintaro Kinugawa, Ryoko Inatome, Shigeru Yanagi
Publikováno v:
iScience, Vol 25, Iss 7, Pp 104582- (2022)
Summary: Abnormal mitochondrial fragmentation by dynamin-related protein1 (Drp1) is associated with the progression of aging-associated heart diseases, including heart failure and myocardial infarction (MI). Here, we report a protective role of outer
Externí odkaz:
https://doaj.org/article/eb77f74353bf4977b20163745c270eb9
Autor:
Suvd Byambaa, Hideki Uosaki, Tsukasa Ohmori, Hiromasa Hara, Hitoshi Endo, Osamu Nureki, Yutaka Hanazono
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 20, Iss , Pp 451-462 (2021)
We conducted two lines of genome-editing experiments of mouse hematopoietic stem cells (HSCs) with the clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein 9 (Cas9). First, to evaluate the genome-editing eff
Externí odkaz:
https://doaj.org/article/2f08446944e44cac965321da03925c00
Autor:
Sean A. Murphy, Matthew Miyamoto, Anaïs Kervadec, Suraj Kannan, Emmanouil Tampakakis, Sandeep Kambhampati, Brian Leei Lin, Sam Paek, Peter Andersen, Dong-Ik Lee, Renjun Zhu, Steven S. An, David A. Kass, Hideki Uosaki, Alexandre R. Colas, Chulan Kwon
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Cardiomyocyte maturation and the acquisition of phenotypes is poorly understood at the single cell level. Here, the authors analyse the transcriptome of single cells from neonatal to adult heart and reveal that peroxisome proliferator-activated recep
Externí odkaz:
https://doaj.org/article/e529861439104233888f042c1cc8bff3
Autor:
William J. Kowalski, Iris H. Garcia-Pak, Wenling Li, Hideki Uosaki, Emmanouil Tampakakis, Jizhong Zou, Yongshun Lin, Kira Patterson, Chulan Kwon, Yoh-Suke Mukouyama
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
Embryos devoid of autonomic innervation suffer sudden cardiac death. However, whether autonomic neurons have a role in heart development is poorly understood. To investigate if sympathetic neurons impact cardiomyocyte maturation, we co-cultured pheno
Externí odkaz:
https://doaj.org/article/14a91070c479432383e8020dc3ae523c
Autor:
Takafumi Miyamoto, Hideki Uosaki, Yuhei Mizunoe, Song-Iee Han, Satoi Goto, Daisuke Yamanaka, Masato Masuda, Yosuke Yoneyama, Hideki Nakamura, Naoko Hattori, Yoshinori Takeuchi, Hiroshi Ohno, Motohiro Sekiya, Takashi Matsuzaka, Fumihiko Hakuno, Shin-Ichiro Takahashi, Naoya Yahagi, Koichi Ito, Hitoshi Shimano
Publikováno v:
Cell Reports: Methods, Vol 1, Iss 4, Pp 100052- (2021)
Summary: Engineered synthetic biomolecular devices that integrate elaborate information processing and precisely regulate living cell behavior have potential in various applications. Although devices that directly regulate key biomolecules constituti
Externí odkaz:
https://doaj.org/article/75ae182478b64fe69033777691d2582b
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
Transcriptome landscape of organs from mice and humans offers perspectives on the process of how organs develop and the similarity and diversity in each organ between the species. Among multi-species and multi-organ dataset, which was previously gene
Externí odkaz:
https://doaj.org/article/8394da84a767448da9d27c6484e0f728
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
Cardiovascular diseases are the leading cause of death worldwide. Therefore, the discovery of induced pluripotent stem cells (iPSCs) and the subsequent generation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) was a pivotal
Externí odkaz:
https://doaj.org/article/78badc492b704f4390e6a64ac826f296
Publikováno v:
Biology, Vol 10, Iss 10, p 981 (2021)
Mitochondrial cardiomyopathy (MCM) is characterized as an oxidative phosphorylation disorder of the heart. More than 100 genetic variants in nuclear or mitochondrial DNA have been associated with MCM. However, the underlying molecular mechanisms link
Externí odkaz:
https://doaj.org/article/ce482b90afa94cf3a6c88b4ae73a76eb