Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Hideki Oishi"'
Publikováno v:
Breeding Science. 60:279-285
Transgenic Italian ryegrass plants expressing the betaine aldehyde dehydrogenase gene of zoysiagrass (ZBD1) were produced via particle bombardment of embryogenic calluses. Although the growth of both non-transgenic and transgenic plants expressing ZB
Publikováno v:
Journal of Plant Physiology. 163:58-68
Summary The replacement histone H3 gene and its 5′-flanking sequence were isolated from Italian ryegrass by polymerase chain reaction and inverse polymerase chain reaction, respectively. Expression analysis showed that this gene is constitutively e
Autor:
Masumi Ebina, Hideki Oishi
Publikováno v:
Journal of Plant Physiology. 162:1077-1086
Summary We isolated cDNAs encoding betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) from the salt-tolerant Poaceae, Zoysia tenuifolia by polymerase chain reactions. Zoysia betaine aldehyde dehydrogenase 1 ( ZBD1 ) is 1892 bp long and codes for 507 a
Publikováno v:
Plant Biotechnology. 19:241-249
We established a microprojectile-bombardment-mediated transformation system in embryogenic calli of Italian ryegrass (Lolium multiflorum Lam.). Bombardment conditions with a PDS-1000/He helium-driven biolistic device were optimized by transient expre
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 62:1633-1636
Yeast had been known to contain only one kind of phospholipid deacylating enzyme with an optimal pH in the acidic range. However, among 8 genera and 25 strains, 7 genera and 13 strains had phospholipid deacylating activity at pH 8.0, when screening o
Publikováno v:
FEMS Microbiology Letters. 145:415-420
An uracil auxotrophic mutant of baker's yeast Torulaspora delbrueckii, which is resistant to 5-fluoro-orotic acid, was complemented by transformation with YEp24 which harbors 2 microns origin and URA3 derived from Saccharomyces cerevisiae. The phosph
Autor:
Yuji Kuwabara, Youichi Tamai, Isao Nakagawa, Yasuo Watanabe, Hideki Oishi, Kenji Yamato, Seiichiro Nagai, Hiroshi Shinmoto
Publikováno v:
NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI. 42:383-387
Publikováno v:
Bioscience, biotechnology, and biochemistry. 63(1)
Phospholipase B (PLB) from the yeast Kluyveromyces lactis was purified to homogeneity from culture medium. The enzyme was highly glycosylated with apparent molecular mass of 160-250 kDa, and had two pH optima, at pH 2.0 and pH 7.5. At acidic pH the e
Publikováno v:
Bioscience, biotechnology, and biochemistry. 60(7)
Phospholipase B from Schizosaccharomyces pombe was purified by ammonium sulfate fractionation and chromatographed on phenyl-Sepharose CL-4B, DEAE-Toyopearl 650M, and TSK gel G4000SW columns. The purified enzyme was a glycoprotein with molecular weigh
Publikováno v:
Analytical chemistry. 59(19)
Previous Li/sup +/-selective polymeric membrane electrodes based on 14-crown-4 derivative 1 have been improved in the Li/sup +/ selectivities against Na/sup +/ and K/sup +/ by using 2 and 3 as the neutral carrier. Examination of the membrane solvents