Zobrazeno 1 - 10
of 2 007
pro vyhledávání: '"Heterologous protein"'
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 426-435 (2024)
Xylanase, an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat, has been found to improve the organizational structure of dough and thus increase its volume. In our past work, one promising xylanase FXYL de
Externí odkaz:
https://doaj.org/article/976a5358e2cf499f944252bb8843ee2f
Autor:
Xiangna Lin, Weiqiu Ding, Shaoyan Zheng, Lianna Wu, Xue Chen, Chunfang Xie, Daling Liu, Dongsheng Yao
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-12 (2024)
Abstract Background Pichia pastoris (Komagataella phaffii) is a promising production host, but the usage of methanol limits its application in the medicine and food industries. Results To improve the constitutive expression of heterologous proteins i
Externí odkaz:
https://doaj.org/article/baed48c0f4fa493584bc2fc2495cae50
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-15 (2024)
Abstract Background The yeast Komagataella phaffii has become a very popular host for heterologous protein expression, very often based on the use of the AOX1 promoter, which becomes activated when cells are grown with methanol as a carbon source. Ho
Externí odkaz:
https://doaj.org/article/8a120628ecf84c8991ece63877d5ed86
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-15 (2024)
Abstract Background The 5´ untranslated region (5´ UTR) plays a key role in regulating translation efficiency and mRNA stability, making it a favored target in genetic engineering and synthetic biology. A common feature found in the 5´ UTR is the
Externí odkaz:
https://doaj.org/article/a18506c7e8cf4b8d8d21ec074347e48a
Autor:
Kamilla Wiull, Live Heldal Hagen, Jelena Rončević, Bjørge Westereng, Preben Boysen, Vincent G. H. Eijsink, Geir Mathiesen
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-17 (2024)
Abstract Background Utilization of commensal bacteria for delivery of medicinal proteins, such as vaccine antigens, is an emerging strategy. Here, we describe two novel food-grade strains of lactic acid bacteria, Lactiplantibacillus pentosus KW1 and
Externí odkaz:
https://doaj.org/article/66d2d3eb94524e709fc19a24101c6ef7
Akademický článek
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Publikováno v:
The Microbe, Vol 3, Iss , Pp 100092- (2024)
The production of heterologous proteins via microbial secretion is currently a prominent focus in biological product development. However, the full realization of this method remains hindered by limited understanding of the protein secretion mechanis
Externí odkaz:
https://doaj.org/article/4491f09af81544eb8fecb0d32ea5c37f
Autor:
Bárbara Ariane Pérez-Fernández, Lisandra Calzadilla, Chiara Enrico Bena, Marco Del Giudice, Carla Bosia, Tammy Boggiano, Roberto Mulet
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Human Embryonic Kidney cells (HEK293) are a popular host for recombinant protein expression and production in the biotechnological industry. This has driven within both, the scientific and the engineering communities, the search for strategies to inc
Externí odkaz:
https://doaj.org/article/aeec90d82b2a4653a44b8b00b4157ba1
Publikováno v:
International Journal of Molecular Sciences, Vol 25, Iss 17, p 9450 (2024)
Transcriptional regulatory networks (TRNs) associated with recombinant protein (rProt) synthesis in Yarrowia lipolytica are still under-described. Yet, it is foreseen that skillful manipulation with TRNs would enable global fine-tuning of the host st
Externí odkaz:
https://doaj.org/article/a3a8e1c36e4c4b3295c61f8924629fce
Publikováno v:
International Journal of Molecular Sciences, Vol 25, Iss 13, p 6957 (2024)
Achieving commercially significant yields of recombinant proteins in Bacillus subtilis requires the optimization of its protein production pathway, including transcription, translation, folding, and secretion. Therefore, in this study, our aim was to
Externí odkaz:
https://doaj.org/article/cd90fa39af364c57a221be080446385c