Generating and validating renewable affimer protein binding reagents targeting SH2 domains.

Autor: Heseltine SJ; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Billenness GJ; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Martin HL; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Tiede C; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Tang AAS; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Foy E; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Reddy G; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Gibson N; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK., Johnson M; Avacta Life Sciences, Wetherby, UK., Webb ME; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK.; School of Chemistry, University of Leeds, Leeds, UK., McPherson MJ; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Tomlinson DC; School of Molecular and Cellular Biology, University of Leeds, Leeds, UK. d.c.tomlinson@leeds.ac.uk.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK. d.c.tomlinson@leeds.ac.uk.

Publikováno v: Scientific reports [Sci Rep] 2024 Nov 16; Vol. 14 (1), pp. 28322. Date of Electronic Publication: 2024 Nov 16.

Affimer proteins are versatile and renewable affinity reagents.

Autor: Tiede C; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Bedford R; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Heseltine SJ; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Smith G; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Wijetunga I; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., Ross R; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., AlQallaf D; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Roberts AP; The Pirbright Institute, Woking, United Kingdom., Balls A; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Curd A; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Hughes RE; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Martin H; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Needham SR; Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Didcot, United Kingdom., Zanetti-Domingues LC; Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Didcot, United Kingdom., Sadigh Y; The Pirbright Institute, Woking, United Kingdom., Peacock TP; The Pirbright Institute, Woking, United Kingdom., Tang AA; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Gibson N; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Kyle H; Avacta Life Sciences, Wetherby, United Kingdom., Platt GW; Avacta Life Sciences, Wetherby, United Kingdom., Ingram N; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., Taylor T; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom., Coletta LP; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., Manfield I; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Knowles M; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., Bell S; Leeds Institute of Biomedical and Clinical Sciences, University of Leeds, Leeds, United Kingdom., Esteves F; Leeds Institute of Cancer Studies and Pathology, University of Leeds, Leeds, United Kingdom., Maqbool A; Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom., Prasad RK; Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom., Drinkhill M; Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom., Bon RS; Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, United Kingdom., Patel V; DSTL Porton Down, Salisbury, United Kingdom., Goodchild SA; DSTL Porton Down, Salisbury, United Kingdom., Martin-Fernandez M; Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Didcot, United Kingdom., Owens RJ; Oxford Protein Production Facility UK, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Didcot, United Kingdom., Nettleship JE; Oxford Protein Production Facility UK, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Didcot, United Kingdom., Webb ME; School of Chemistry, University of Leeds, Leeds, United Kingdom., Harrison M; School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom., Lippiat JD; School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom., Ponnambalam S; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Peckham M; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Smith A; Avacta Life Sciences, Wetherby, United Kingdom., Ferrigno PK; Avacta Life Sciences, Wetherby, United Kingdom., Johnson M; Avacta Life Sciences, Wetherby, United Kingdom., McPherson MJ; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom., Tomlinson DC; School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.; Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, United Kingdom.

Publikováno v: ELife [Elife] 2017 Jun 27; Vol. 6. Date of Electronic Publication: 2017 Jun 27.

Affimer sandwich probes for stable and robust lateral flow assaying.

Autor: Goyal, Garima^1,2,3 (AUTHOR), Sharma, Antareep^1,2,3 (AUTHOR), Tok, Alfred Iing Yoong³ (AUTHOR), Palaniappan, Alagappan^2,3 (AUTHOR) alps@ntu.edu.sg, Liedberg, Bo^1,2,3 (AUTHOR) bliedberg@ntu.edu.sg

Publikováno v: Analytical & Bioanalytical Chemistry. Jun2022, Vol. 414 Issue 14, p4245-4254. 10p.

Targeting platelet inhibition receptors for novel therapies: PECAM-1 and G6b-B.

Autor: Soriano Jerez, Eva M^1,2 (AUTHOR) e.sorianojerez@pgr.reading.ac.uk, Gibbins, Jonathan M¹ (AUTHOR), Hughes, Craig E¹ (AUTHOR)

Publikováno v: Platelets. 2021, Vol. 32 Issue 6, p761-769. 9p.

Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests.

Autor: Álvarez-Rodríguez, Beatriz¹ (AUTHOR), Tiede, Christian¹ (AUTHOR), Hoste, Alexis C. R.^1,2 (AUTHOR), Surtees, Rebecca A.¹ (AUTHOR), Trinh, Chi¹ (AUTHOR), Slack, Gillian S.³ (AUTHOR), Chamberlain, John³ (AUTHOR), Hewson, Roger³ (AUTHOR), Fresco, Alba² (AUTHOR), Sastre, Patricia² (AUTHOR), Tomlinson, Darren C.¹ (AUTHOR), Millner, Paul A.¹ (AUTHOR), Edwards, Thomas A.¹ (AUTHOR) t.a.edwards@leeds.ac.uk, Barr, John N.¹ (AUTHOR) t.a.edwards@leeds.ac.uk

Publikováno v: PLoS Neglected Tropical Diseases. 6/3/2020, Vol. 14 Issue 6, p1-20. 20p.