Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Herman G P, Swarts"'
Autor:
Herman G P, Swarts, Jan B, Koenderink
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1377
ATP provides the energy that is essential for all P-type ATPases to actively transport their substrates against an existing gradient. This ATP hydrolysis can be measured using different methods. Here, we describe a method that uses radiolabeled [γ-(
Autor:
Jan B, Koenderink, Herman G P, Swarts
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1377
P-type ATPases can be expressed in several cell systems. The baculovirus expressions system uses an insect virus to enter and express proteins in Sf9 insect cells. This expression system is a lytic system in which the cells will die a few days after
Autor:
Herman G. P. Swarts, Jenny VanOostrum, Venkata R. P. Ratnala, Remko A. Bakker, Rob Leurs, Willem J. DeGrip, Huub J. M. DeGroot
Publikováno v:
European Journal of Biochemistry. 271:2636-2646
This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamine H1 receptor was used to
Autor:
Karl M. Weigand, Mette Laursen, Herman G. P. Swarts, Anthonius H. J. Engwerda, Christian Prxfdfert, Julia Sandrock, Poul Nissen, Natalya U. Fedosova, Frans G. M. Russel, Jan B. Koenderink
Publikováno v:
Chemical Research in Toxicology.
Autor:
Venkata R P, Ratnala, Herman G P, Swarts, Jenny, VanOostrum, Rob, Leurs, Huub J M, DeGroot, Remko A, Bakker, Willem J, DeGrip
Publikováno v:
European journal of biochemistry. 271(13)
This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamine H1 receptor was used to